This experiment investigates the effects of adding different concentrations of lysine to feed on the growth performance and immune function of Cherax quadricarinatus.This experiment selected 900 healthy Cherax quadri-carinatus with an initial weight of(0.45±0.32)g as the experimental subjects,divided into 5 experimental groups(A-E),each with 3 replicates.Five types of formula feed with different amounts of lysine added:1.3%,1.6%,1.9%,2.2%,and 2.5%of equal nitrogen and equal energy were prepared for an 8-week feeding experi-ment.The experimental results showed that compared with other groups,Group D(2.2%)reached its maximum value.There were significant differences in final body weight,weight gain rate,and specific growth rate(P<0.05).The gene expression levels of HEM,ALB,GLB in serum,AKP,ACP in liver and pancreas,and immune genes ALF,LZM,CHH,and SPI in group D reached their maximum values,with significant differences(P<0.05);The activity of ALT and AST in serum and hepatopancreas in group D reached the minimum value,with significant differences(P<0.05).In summary,this article recommends that the optimal amount of lysine added to Cherax quadricarinatus feed is 1.75%-2.05%.
lysineCherax quadricarinatusgrowth performanceimmune function