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中草药对草鱼机体免疫力的影响

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为研究中草药对草鱼(Ctenopharyngodon idella)机体免疫力的影响,且确定中草药在草鱼饲料中的最适添加水平,选择120尾体重约为100g的健康草鱼,按照单因素试验设计,均分4个处理,每个处理3个重复,每个重复10尾草鱼.各组草鱼分别投喂含有0%(对照组)、1%、2%和4%的中草药的饲料,饲养8周.试验结束后,每个重复组挑选4尾草鱼采集血液,检测血液免疫指标.结果显示,在草鱼饲料中添加2%的中草药可以提高血清中溶菌酶活力(P<0.05);添加1%和2%的中草药可以显著提高血清中超氧化物歧化酶活力(P<0.05),显著降低了丙二醛水平(P<0.05).研究表明,在草鱼饲料中添加适量的中草药可以提高机体的非特异性免疫力,且综合成本及各项指标数据确定中草药的最适添加水平为2%.
Effects of Chinese herbs on organismal immunity of Ctenopharyngodon idellus
In order to study the effects of Chinese herbs on the immunity of Ctenopharyngodon idella and to deter-mine the optimum level of Chinese herbs in Ctenopharyngodon idella feed.In this study,120 healthy grass carp weighing about 100 g were selected and divided into four treatments with three replicates of 10 fish each according to a one-way experimental design.Ctenopharyngodon idella in each group were fed with diets containing 0%(con-trol),1%,2%and 4%of Chinese herbs for 8 weeks.At the end of the experiment,four Ctenopharyngodon idella from each replicate group were selected for blood collection and blood immunity indexes were tested.The results showed that the addition of 2%of the herbs to the Ctenopharyngodon idella feed increased the serum lysozyme activ-ity(P<0.05);the addition of 1%and 2%of the herbs significantly increased the serum superoxide dismutase ac-tivity(P<0.05),and significantly reduced the malondialdehyde level(P<0.05).The study showed that the addi-tion of appropriate amount of Chinese herbs to Ctenopharyngodon idella feed can improve the non-specific immunity of the organism,and the optimum level of addition of Chinese herbs was determined to be 2%by combining the cost and data of various indexes.

Chinese herbsimmunityCtenopharyngodon idella

方进林、钱涛

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开化佳艺家庭农场,浙江衢州 324305

开化县水产技术推广站,浙江衢州 324300

中草药 免疫力 草鱼(Ctenopharyngodon idella)

2024

黑龙江水产
黑龙江省渔业经济研究所 黑龙江省渔业协会

黑龙江水产

影响因子:0.13
ISSN:1674-2419
年,卷(期):2024.43(1)
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