Effects of feed supplementation with chitosan on the non-specific immune function of Carassius auratus gibelio
The aim of the experiment was to investigate the effects of adding different levels of Chitosan oligosaccha-ride([3-1,4-oligosaccharide-glucosamine)to the feed of Carassius auratus gibelio on its non-specific immune func-tion.A total of 480 healthy Carassius auratus gibelio weighing about 50 g were randomly divided into four treatments,with three replicates of 40 Carassius auratus gibelio in each treatment.Each group of Carassius auratus gibelio was fed with test baits containing 0％(control group),0.25％,0.50％and 0.75％chitosan,respectively.The experimental period was 60 day.The results showed that compared with the control group,when 0.50％and 0.75％chitosan was added to the heterotrophic crucian carp culture,the serum malondialdehyde level was significantly reduced(P＜0.05);serum lysozyme activity and superoxide dismutase activity were significantly higher than those in the control group and the group with 0.25％chitosan(P＜0.05);and serum catalase activity was significantly higher than that in the control group(P＜0.05).Studies have shown that the addition of appropriate amount of chitosan in Carassius au-ratus gibelio culture production can significantly improve the non-specific immune function of the organism,and the optimal amount of chitosan is 0.50％to 0.75％considering the comprehensive cost of culture.
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维普
