Effects of different levels of Bacillus subtilis on immunity of Litopenaeus van-namei
In order to explore the effects of different levels of Bacillus subtilis on the nonspecific immunity of the organ-ism of Litopenaeus vannamei,600 healthy Litopenaeus vannamei weighing about 0.45 g were selected for this experi-ment,which were divided into four groups of three replicates each according to a one-way experimental design,with each replicate of each group was divided into four groups with three replicates,each with 50 shrimps.Each group was fed a basal diet containing 0%(control),0.5%,1.0%and 1.5%Bacillus subtilis,respectively.After 8 weeks of feeding,blood was collected and tested for serum non-specific immunity indexes.The results showed that lysozyme,alkaline phosphatase,acid phosphatase,and phenoloxidase activities were significantly higher(P＜0.05)in the ser-um of Litopenaeus vannamei in test group 2 compared with the control group,and catalase and superoxide dismutase activities were significantly higher(P＜0.05)in the serum of Litopenaeus vannamei in both test group 2 and test group 3.The study showed that the addition of a certain dose of Bacillus subtilis in the production of Litopenaeus vannamei culture can improve the non-specific immunity of the organism,and the appropriate amount is 1.0%.
万方数据
维普
