In order to study the effects of water temperature and drug dosage on artificial oxytocin of Mystus gutta-tus,275 Mystus guttatus were injected with oxytocin drugs DOM,LRH-A2 and HCG at different water temperatures from 25℃±1℃to 30℃±1℃.The results showed that the optimal dose of oxytocin was DOM 7 mg+LRH-A2 9 μg and(DOM)7 mg+(LRH-A2)9 μg+(HCG)550 IU at 28℃±1℃,and the optimal dose increased by 8% at 26℃±1℃~27℃±1℃.Under the temperature of 29℃±1℃~30℃±1℃,the effect of reducing the dose by 8%was the most obvious.The results showed that the highest rate of oxytocin was found at 28℃~29℃,and either too high or too low temperature would decrease the rate of oxytocin.The temperature of 25℃±1℃ and below is not suitable for oxytocin.