Effects of water temperature and drug dose on artificial oxytocin of Mystus maculatus
In order to study the effects of water temperature and drug dosage on artificial oxytocin of Mystus gutta-tus,275 Mystus guttatus were injected with oxytocin drugs DOM,LRH-A2 and HCG at different water temperatures from 25℃±1℃to 30℃±1℃.The results showed that the optimal dose of oxytocin was DOM 7 mg+LRH-A2 9 μg and(DOM)7 mg+(LRH-A2)9 μg+(HCG)550 IU at 28℃±1℃,and the optimal dose increased by 8% at 26℃±1℃~27℃±1℃.Under the temperature of 29℃±1℃~30℃±1℃,the effect of reducing the dose by 8%was the most obvious.The results showed that the highest rate of oxytocin was found at 28℃~29℃,and either too high or too low temperature would decrease the rate of oxytocin.The temperature of 25℃±1℃ and below is not suitable for oxytocin.