Oxidation resistance and salt resistance analysis of Thioredoxin peroxidase (BvM14-Tpx) gene from Sugar Beet M14 line in transgenic prokaryotic and eukaryotic cells
Sugar beet monosomic addition line M14 was obtained from the intercross between a cultivated species Beta vulgaris L .and a wild species Beta corollif lora Zoss . It contains the Beta vulgaris L . genome with the addition of chromosome 9 of Beta corollif lora Zoss . The M14 line has exhibited the characteristics of stress tolerance and apomixes .Previously ,a cDNA whole length of the BvM14-Tpx gene which coded for thioredoxin peroxidase was obtained by RACE .It was reported that the Tpx gene was involved in the removal of the various peroxide reaction so as to improve the resistance of plants . In this study ,the E.coli expression system of BvM14-Tpx gene was built . The growth curve of E.coli BL21 (DE3) bacteria transferring BvM14-Tpx gene was measured under the stress of H2O2 .The results showed that transgenic E. coli strains entered growing period 1 h earlier than the control strains , suggesting that BvM14-Tpx genes can ease the inhibition of H2O2 on the growth of E.coli .And the eukaryotic expression system of BvM14-Tpx gene was built . The results of transgenic Saccharomyces cerevisiae under H2O2 and NaCl stress , showed that transgenic yeast grown better than the control , suggesting that Bv M14-T p x can improve the salt tolerance ability of transgenic yeast .
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