甜菜M 14品系是由栽培甜菜和野生白花甜菜杂交后获得的带有野生白花甜菜第9号染色体的单体附加系，具有抗逆、无融合等优良特性。在前期工作中，对盐胁迫下叶片膜蛋白进行了定量蛋白质组学分析，与未处理对照相比，200、400 m M盐胁迫下共获得50个差异蛋白质点，选择其中3个蛋白质点为研究对象，与实验室前期相同盐处理下获得的转录组数据库相匹配，获得基因cDNA全长，将此3个基因命名为 BvM14‐A T‐PaseF、 BvM14‐ATPaseH 和 BvM14‐PsaD ；进行在线功能预测；设计引物，对盐胁迫（0、200、400 mM）处理下此3个基因进行实时荧光定量分析，了解在盐胁迫下基因转录与蛋白水平上表达量的关系。结果表明，与未处理对照相比，200、400 mM 盐浓度处理下 BvM14‐ATPaseF基因分别上调3.70与4.47倍， BvM14‐AT‐PaseH 基因在盐胁迫处理下分别上调0.85与1.36倍，而 BvM14‐PsaD 基因随着盐浓度的增加却下调0.97与2.54倍，表明在盐胁迫下不同基因具有的功能不同，而导致转录水平与蛋白水平的表达量并不完全相同。
Specific expression analysis of BvM14-ATPaseF、 BvM14-ATPaseH and BvM14-PsaD under salt stress of sugar beet M14 lines
Sugar beet M14 line is a unique germplasm that contains genetic materials from Beta vulgaris L and Beta corolliflora Zoss and exhibits tolerance to salt stress .In previous work ,it was focusing on analyzing proteomic protein quantitative of the leaf membrane .Under salt stress ,50 proteins exhibited differential protein level changes .We regard 3 of 50 as research object to get full length of genes with prediction function online ,and design primers by using the transcriptome data .Using Real‐time PCR to analysis the expression of the genes which under the treatment of salt stress (0 ,200 ,400 mM ) .The result shows that ,the BvM14‐A T PaseF gene is up‐regulated 3.7 and 4.47 respectively compared with control .And the expression of BvM14‐A T PaseH gene is also up‐regulated 0.85 and 1.36 . However , different results appear in BvM14‐PsaD gene ,0.97 down‐regulations in 200 mM and 2.54 in 400 mM salt stress processing .The function of gene is not consistent so that the level of transcription is not entirely consistent with the protein level . Therefore ,M14 has been used as an abundant genetic resource for isolating valuable genes in w ild species .
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