Effects of mannans substrates on the growth and mannanase production of Lactobacillus casei HDS-01
Mannanase derived mainly from microorganisms has been widely used in various industrial fields such as textile,medicine and food processing.Mannanase produced by lactic acid bacteria(LAB)can hydrolyze theβ-1,4-glycosidic bonds in mannans to prepare mannan oligosaccharides(MOS)which is potential prebiotics with high bio-safety.In order to enhance the LAB mannanase-producing level,this study investigated the effects of three mannans substrates,i.e.Konjac Glucomannan(KGM),locust bean gum(LBG)and Guar gum(GUAR)on growth,enzyme production and MOS preparation of Lactobacillus casei HDS-01.When L.casei HDS-01 was cultured in the KGM media containing 0.2％glucose,the best fermentation results was obtained.The highest biomass reached 9.41 Log(CFU·mL-1)and the mannanase activity was 33.16±0.92 U·mL-1.The High Pressure Liquid Chromatography(HPLC)was used to determine the MOS concentration according peak area.The maximum concentration of mannobiose,mannotriose and mannotetraose was 0.63±0.005,0.32±0.020 and 0.26±0.019 mg·mL-1,respectively.The approach set a basis for the large-scale mannanase production by L.casei HDS-01 and also laid a foundation of MOS preparation.
NETL
NSTL
万方数据
