首页|扎龙湿地野大麦对NaCl胁迫的生理响应

扎龙湿地野大麦对NaCl胁迫的生理响应

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为了研究野大麦对NaCl胁迫的响应, 试验采用室内盆栽幼苗培养和理化分析的方法, 分别以不同浓度NaCl[1% (T1) 、2% (T2) 、3% (T3) 、4% (T4) 、5% (T5) ]进行胁迫处理, 测定野大麦幼苗叶片生理生化指标.结果表明:随着NaCl浓度的升高, 叶片相对电导率及丙二醛、叶绿素、Na+含量呈升高趋势, T5组最高, 分别为98.4%、47.55μmol/L、5.10 mg/g和66.84 mg/g;可溶性蛋白含量及过氧化物酶 (POD) 活性呈先增高后降低的趋势, T2组最高, 分别为76.24μg/g和16 800 U/ (g·min);K+含量变化趋势不明显, 基本维持同一水平;Na+/K+比值呈升高趋势, T5组最高.说明野大麦耐受NaCl能力较强, 能够通过多种代谢方式适应盐胁迫环境.
The physiological response of Hordeum brevisubulatum to NaCl stress in Zhalong Wetland
In order to study the response of Hordeum brevisubulatum to NaCl stress, the physiological and biochemical indexes of Hordeumbrevisubulatum seedlingleavesunder the NaCl stress of different concentrations (1% [T1], 2% [T2], 3% [T3], 4% [T4], 5% [T5]) were determined by using indoor potted seedling cultivation and physicochemical analysis, respectively. The results showed that with the increase of NaCl concentration, the relative conductivity and the contents of malondialdehyde, chlorophyll and Na+showed a trend of increase, and the maximum values in T5 group were 98. 4%, 47. 55 μmol/L, 5. 10 mg/g and 66. 84 mg/g, respectively. The content of soluble protein and the activity of peroxidase POD increased first and then decreased, and the maximum values in T2 group were 76. 24 μg/g and 16 800 U/ (g·min), respectively.The change trend of K+was not obvious and maintainedthe same level. The ratio of Na+/K+increased and the maximum value appeared in T5 group.The results indicated that Hordeum brevisubulatum had high tolerance to NaCl and could adapt to salt stress by various metabolic methods.

Hordeum brevisubulatum (Trin.) Linkprolinesoluble proteinmalondialdehydechlorophyllPOD activity

焦德志、宋士伟

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齐齐哈尔大学生命科学与农林学院/抗性基因工程与寒地生物多样性保护黑龙江省重点实验室,黑龙江齐齐哈尔 161006

野大麦 脯氨酸 可溶性蛋白 丙二醛 叶绿素 POD活性

国家自然科学基金齐齐哈尔大学研究生创新科研项目

31672471YJSCX2017-028X

2018

黑龙江畜牧兽医
黑龙江省畜牧兽医学会

黑龙江畜牧兽医

北大核心
影响因子:0.402
ISSN:1004-7034
年,卷(期):2018.(8)
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