Study on the effects of Nesfatin-1 on glycolipid metabolism in obese rats
In order to explore the effects of Nesfatin-1 on glucose and lipid metabolism in obese rats,40 healthy SPF SD rats aged eight weeks were randomly divided into control group(n=8)and model group(n=32).The control group was fed a maintenance diet,while the model group was fed a high-fat diet for 10 weeks.When the body weight of the model group(under fasting condition)increased by more than 20%compared with that of the control group,the modeling was considered successful.The successfully modeled rats were further randomly divided into model control group(n=8)and model experimental group,which was subdivided into Nesfatin-1 low-dose group(n=8,1 μg/kg),Nesfatin-1 medium-dose group(n=8,10 μg/kg)and Nesfatin-1 high-dose group(n=8,100 μg/kg).The experimental groups received daily tail vein injections with 1,10,100 μg/kg respectively once a day for 5 weeks,while the control group and the model control group were injected with normal saline at the weight of 10 mL/kg once a day for 5 weeks.At the end of the experiment,serum FFA,TC,TG,TNF-α and IL-10 concentrations in each group were detected by ELISA,and liver tissues of each group were collected and weighed.Real-time quantitative PCR was used to detect miR-122 and miR-802 related to fat metabolism in liver as well as the expression level of UCP-1 gene mRNA in scapularadipose tissue of each group.UCP-1 protein concentration in the scapular adipose tissue was determined by BCA method in the control,model control group,and model experimental groups that had significant differences in UCP-1 mRNA expression level.The results showed that,compared with the control group,the serum FFA,TC,TG,TNF-α concentrations,liver weight and the relative expression levels of miR-122 and miR-802 in liver tissue were significantly increased in model control group(P<0.05),while the serum IL-10 concentration and UCP-1 mRNA levels in scapular adipose tissue were significantly decreased(P<0.05).There was no significant difference in UCP-1 protein expression in adipose tissue(P>0.05).Compared with model control group,the serum FFA concentration in Nesfatin-1 low dose group was significantly decreased(P<0.05),while no significant differences were observed in Nesfatin-1 medium and high dose groups(P>0.05).The serum TC concentration in all Nesfatin-1 groups was significantly decreased(P<0.05).The serum TG concentration in Nesfatin-1 medium and high dose groups was significantly decreased(P<0.05),with no significant difference in Nesfatin-1 low dose group(P>0.05).The serum TNF-α concentration was significantly decreased(P<0.05)and IL-10 concentration was significantly increased(P<0.05)in all Nesfatin-1 groups.The liver weight of Nesfatin-1 medium and high dose groups was significantly decreased(P<0.05),while no significant difference was observed in Nesfatin-1 low dose group(P>0.05).The relative expression levels of miR-122 in liver tissues were significantly decreased in Nesfatin-1 low and high dose groups(P<0.05),with no significant difference in Nesfatin-1 medium dose group(P>0.05).The relative expression levels of miR-802 in liver tissues were significantly decreased in low,medium and high dose Nesfatin-1 groups(P<0.05).The relative expression level of UCP-1 gene mRNA in adipose tissue was significantly increased in Nesfatin-1 high dose group(P<0.05),while no significant differences were observed in Nesfatin-1 low and medium dose groups(P>0.05).The UCP-1 protein expression level in adipose tissue was significantly increased in Nesfatin-1 high dose group(P<0.05).These results indicated that Nesfatin-1 could promote the metabolism of glucose and lipid in the body.
万方数据
维普
