Effects of selenomethionine on preservation of boar semen at room temperature
In order to improve the quality of porcine sperm and prolong survival time during room temperature preservation,semen from 8 Duroc boars was collected.Selenomethionine(SeMet)of different concentrations(0,0.1,1 and 10 mg/L)was added to the diluent of pig semen stored at room temperature and the oxidative damage model of hydrogen peroxide(H2 O2)was constructed.The motility,vitality,plasma membrane integrity and acrosome integrity of porcine sperm at 1,3,5,7,9 days after storage at room temperature and the activities of glutathione peroxidase(GSH-Px,)superoxide dismutase(SOD)and catalase(CAT),the content of malondialdehyde(MDA)and reactive oxygen species(ROS)in spermatozoa under oxidative stress conditions were detected.The results showed that the sperm vitality and motility in the C group(1 mg/L SeMet)were significantly increased(P<0.05),and the plasma membrane and the acrosome integrity of sperm were significantly enhanced(P<0.05)when stored at room temperature for 5～9 days.In the oxidative damage model,H2 O2 supplementation significantly increased the contents of MDA and ROS in the sperm(P<0.05),but the activities of GSH-Px and SOD in porcine sperm were significantly increased after SeMet treatment(P<0.05),while the contents of MDA and ROS were significantly decreased(P<0.05).The results indicated that adding 1 mg/L SeMet into diluent of pig semen stored at room temperature could significantly enhance the activities of antioxidant enzymes in pig semen,and thus improve the quality and storage time of pig semen.
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