In order to study the origin,evolution and adaptability of Zhijin white geese to determine the whole genome sequencing and assembly strategy suitable for this species,and evaluate Zhijin white goose genome size and heterozygosity through low coverage genome Survey sequencing to determine the suitable whole genome sequencing strategy for Zhijin white goose,in this study,the genomic information such as genome size,heterozygosity,repeat sequence and GC content of Zhijin white geese was studied by Illumina HiseqTM 2000(the second generation of high-throughput sequencing).The results showed that 66.16 Gb genome sequencing data of Zhijin white geese was obtained;the estimated genome size was about 1.11 Gb;the sequencing depth was 59.57X,and GC content was 42.08%.From the K-mer distribution curve,it was found that there was an obvious heterozygosity peak in the genome of Zhijin white geese,with a heterozygosity rate of 0.62%and a repeat sequence content of 11.44%.The plastid of Zhijin white goose was evaluated,and it was found that the extracellular DNA content was very low.The results of this study were of great significance to reveal the origin,evolution and essential information of Zhijin white geese,and provide a theoretical basis for the follow-up study of species evolution,ploidy level and whole genone sequencing of Zhijin White geese.
Zhijin white geesegenomesurveyheterozygosityGC contentgenome size
万方数据
维普
