摘要
为了解信阳地区鹅源大肠杆菌的系统进化分群、毒力基因携带情况和耐药情况,试验从信阳地区部分养殖户送检的 46 只疑似大肠杆菌感染病鹅的肝脏、脾脏等组织中进行大肠杆菌的分离、鉴定,并对分离菌株进行系统进化分群、毒力基因检测、耐药性分析、耐药基因检测.结果表明:共分离到 23 株大肠杆菌,分别命名为GE1～GE23.23 株大肠杆菌在 4 种系统进化群中均有一定比例的分布,其中A群有 3 株(GE11、GE12、GE19,占比为 13.04%),B1 群有3 株(GE4、GE15、GE23,占比为 13.04%),B2 群有 9 株(GE1、GE2、GE8、GE10、GE14、GE16、GE17、GE20、GE21,占比为39.13%),D群有8 株(GE3、GE5、GE6、GE7、GE9、GE13、GE18、GE22,占比为34.78%).在毒力基因检测中,23 株大肠杆菌marA、fimC、fyuA、iroN、iss、hlyF、ompT、iutA 和 irp2 基因的检出率分别为 100%、95.65%、52.17%、47.83%、43.48%、39.13%、34.78%、30.43%和 17.39%.23 株分离菌对阿莫西林/克拉维酸钾的耐药率最高,为 65.22%;然后依次为磺胺甲口恶唑/甲氧苄啶、强力霉素、氟苯尼考、多黏毒素B、头孢噻呋和左氧氟沙星、环丙沙星,耐药率分别为56.52%、52.17%、47.83%、34.78%、17.39%和 17.39%、8.70%.23 株分离菌对亚胺培南的敏感率最高,为 100%;然后依次为丁胺卡那、链霉素、新霉素、环丙沙星和左氧氟沙星、头孢噻呋、氟苯尼考、多黏菌素B、强力霉素、磺胺甲口恶唑/甲氧苄啶,敏感率分别为 82.61%、82.61%、47.83%、47.83%和 47.83%、34.78%、26.09%、21.74%、17.39%、8.70%.23 株分离菌中有12 株表现为多重耐药,其中6,7 耐菌株各有1 株,占比为4.35%;4,5 耐菌株各有3 株,占比为 13.04%;3 耐菌株有 4 株,占比为 17.39%.在耐药基因检测中,tetA基因的检出率最高,为 82.61%;然后依次为folR、sul2、aadA、blaTEM、blaCTX-M、aphA1、clmA、sul3、sul1、aphA2,检出率分别为 73.91%、65.22%、56.32%、52.17%、47.83%、47.83%、43.48%、26.09%、13.04%和 13.04%.说明信阳地区鹅源大肠杆菌B2 群和D群占比较大,携带多种毒力基因和耐药基因,且多表现为多重耐药.
Abstract
In order to understand the phylogenetic clustering,virulence gene carrying and drug resistance of goose-derived E.coli in Xinyang area,in the experiment,isolation and identification of E.coli were carried out from the liver,spleen and other tissues of 46 suspected E.coli infected geese sent by some farmers in Xinyang area;the isolated strains were carried out for systematical clustering,virulence gene detection,drug resistance analysis,and drug resistance gene detection.The results showed that a total of 23 strains of E.coli were isolated and named GE1-GE23.The 23 strains of E.coli were distributed in a certain proportion of the four phylogenetic groups,including 3 strains in group A(GE11,GE12,GE19,accounting for 13.04%),3 strains in group B1(GE4,GE15,GE23,accounting for 13.04%),9 strains in group B2(GE1,GE2,GE8,GE10,GE14,GE16,GE17,GE20,GE21,accounting for 39.13%),and 8 strains in group D(GE3,GE5,GE6,GE7,GE9,GE13,GE18,GE22,accounting for 34.78%).The detection rates of marA,fimC,fyuA,iroN,iss,hlyF,ompT,iutA and irp2 genes in 23 strains of Escherichia coli were 100%,95.65%,52.17%,47.83%,43.48%,39.13%,34.78%,30.43%and 17.39%,respectively.The 23 isolates had the highest resistance rate to amoxicillin/clavulanate potassium,which was 65.22%.Then sulfamethoxazole/trimethoprim,doxycycline,florfenicol,polymyxoxin B,ceftiofur,levofloxacin and ciprofloxacin were followed,and the drug resistance rates were 56.52%,52.17%,47.83%,34.78%,17.39%,17.39%and 8.70%,respectively.The 23 isolates had the highest susceptibility rate to imipenem,which was 100%.Then amicana,streptomycin,neomycin,ciprofloxacin and levofloxacin,cefotaxifur,florfenicol,polymyxin B,doxycycline,sulfamethoxazole/trimethoprim were followed,and the sensitivity rates were 82.61%,82.61%,47.83%,47.83%and 47.83%,34.78%,26.09%,21.74%,17.39%and 8.70%,respectively.Among the 23 isolates,12 strains showed multi-drug resistance,of which one strain was 6-resistant and one was 7-resistant,each accounting for 4.35%.There were three 4-resistant and 5-resistant strains,each accounting for 13.04%;there were four 3-resistant strains,accounting for 17.39%.In the detection of drug resistance genes,the detection rate of tetA gene was the highest,which was 82.61%;then followed by folR,sul2,aadA,blaTEM,blaCTX-M,aphA1,clmA,sul3,sul1 and aphA2,which were detected at 73.91%,65.22%,56.32%,52.17%,47.83%,47.83%,43.48%,26.09%,13.04%and 13.04%,respectively.The results indicated that the B2 and D groups of E.coli from goose in Xinyang area accounted for a large proportion,carrying a variety of virulence genes and drug resistance genes,and most of them showed multi-drug resistance.
国家科技期刊平台
NSTL
维普
万方数据