Objective:To compare and analyze the diagnostic value of biochemical test and urine test in diabetes.Methods:163 suspected patients with diabetes who were diagnosed in our hospital from January 2022 to March 2023 were included in the observation group,and 147 health examinees who were examined in our hospital were included in the control group.The subjects of the two groups were subjected to biochemical tests and urine tests.The results of urine sugar and blood sugar tests of the two groups were compared.With the results of oral glucose tolerance test as the gold standard,the diagnostic efficacy of biochemical tests and urine tests in diabetes were compared and analyzed.Result:The levels of urine glucose and glycated hemoglobin(HbAlc),fasting blood glucose(FBG),and 2-hour postprandial blood glucose(2hPG)in the observation group were significantly higher than those in the control group,and the differences in urine glucose and blood glucose levels between the two groups were statistically significant(P<0.05).Compared with urine test,the diagnostic sensitivity,specificity,accuracy and negative predictive value of biochemical test in diabetes were significantly higher.The diagnostic efficacy of the two test methods was statistically significant(P<0.05),and the positive predictive value of biochemical test and urine test was statistically significant(P<0.05).Conclusion:Compared with urine test,biochemical test has significantly higher diagnostic value in diabetes.It can improve the diagnostic efficacy of glucose tolerance,effectively control the risk of missed diagnosis and misdiagnosis,and provide guidance and reference for clinical intervention plans and programs.