Optimization of liquid fermentation condition of Bacillus natto NK4 for nattokinase production
To improve the production of nattokinase,single-factor experiments and orthogonal experiments were conducted to optimize the fermentation conditions of Bacillus natto NK4.The optimal conditions for liquid fer-mentation of this strain were obtained.The results showed that the optimal fermentation conditions for Bacillus natto to produce nattokinase were an inoculum size of 2%,an initial pH of 6.0,a fermentation temperature of 34℃,and a fermentation time of 72.0 h.Under these conditions,the enzyme activity of nattokinase increased from 848.28 IU/mL to 1 569.31 IU/mL.
国家科技期刊平台
NSTL
万方数据
维普
