首页|香菇中呈味核苷酸提取工艺优化

香菇中呈味核苷酸提取工艺优化

扫码查看
香菇含有的呈味核苷酸可增加食物的甜味,减少鱼腥味和苦味等,显著提升口感,广泛用于肉类、甜点等食品加工领域.以呈味核苷酸得率为评价指标,采用微波-超声辅助纤维素酶法提取香菇中的呈味核苷酸,分别探究了微波时间、超声时间、酶加入量、pH值和酶解时间对呈味核苷酸得率的影响.在此基础上设计正交实验,优化提取工艺.结果表明,在微波处理时间70 s、超声波处理时间40 min、纤维素酶加入量6 000 U/g、pH值5.0,酶解时间3h的条件下,呈味核苷酸的得率达到4.13%.
Extraction technology of flavored nucleotides in lentinula edodes
The umami nucleotides in lentinula edodes can enhance the sweetness of food,reduce the fishy and bitter tastes,and significantly improve the taste.They are widely used in the processing of meat,desserts,and other food products.In this study,the extraction of umami nucleotides from lentinula edodes was evaluated u-sing the yield of umami nucleotides as the evaluation index.The ultrasound-assisted and microwave-assisted cellulase methods were used to extract umami nucleotides from lentinula edodes,and the optimal extraction process was explored.The effects of microwave time,ultrasound time,enzyme addition amount,pH value,and enzymatic hydrolysis time on the yield of umami nucleotides were investigated.Based on this,an orthogonal ex-periment was designed to optimize the extraction process.The results showed that under the conditions of mi-crowave treatment for 70 seconds,ultrasound treatment for 40 minutes,cellulase addition amount of 6 000 U/g,pH value of 5.0,and enzymatic hydrolysis time of 3 hours,the yield of umami nucleotides reached 4.13%.

lentinula edodesultrasoundmicrowaveflavored nucleotidescellulase

刘俊红、徐佳丽、马亚娜、叶延欣

展开 >

河南城建学院 生命科学与工程学院,河南 平顶山 467036

香菇 超声波 微波 呈味核苷酸 纤维素酶

河南省高等学校重点科研项目

20A550001

2024

河南城建学院学报
河南城建学院

河南城建学院学报

影响因子:0.457
ISSN:1674-7046
年,卷(期):2024.33(2)
  • 17