Exploring the mechanism of homotherapy for heteropathy between bronchial asthma and ulcerative colitis based on bioinformatics
Objective:To identify the potential shared biomarkers between bronchial asthma(asthma)and ulcerative colitis(UC)through bioinformatics analysis and explore their common pathogenic mechanism.Methods:The datasets GSE76262,GSE206285,GSE69683,and GSE87466 containing samples from 529 asthma patients,637 UC patients,and 147 healthy controls,were downloaded from GEO database.The datasets GSE76262 and GSE206285 were used as the training datasets,while GSE69683 and GSE87466 were used as independent validation datasets.The differentially expressed genes(DEGs)of the samples from asthma,UC patients and healthy controls were screened by limma R software package,and the common DEGs of asthma and UC were obtained by taking the intersection of the two disease-related DEGs.A protein-protein interaction(PPI)network was constructed to identify key biomarkers shared between asthma and UC.The cytoHubba algorithm was used to select core biomarkers with high degree within the PPI network.Functional annotation,enrichment analysis,and expression validation were conducted to gain insights into the biological functions of these biomarkers and their relevance to both diseases.Results:A total of 209 common DEGs were identified between asthma and UC,comprising 112 upregulated genes and 97 downregulated genes.MMP9,CCL2,SELL,CCR7,ICAM1,ITGAX,NFKBIA,CXCR4,AGPS,and TLR2 were identified as core biomarkers shared between asthma and UC.RELA and NFKB1 are key transcription factors in this context.Functional enrichment analysis showed that these DEGs were mainly involved in chemotactic and cytokine activities as well as lipid and atherosclerosis.Conclusion:The genes MMP9,CCL2,SELL,CCR7,ICAM1,ITGAX,NFKBIA,CXCR4,AGPS,and TLR2 represent potential core genes implicated in the pathogenesis of asthma and UC.The comorbid mechanisms of asthma and UC may involve chemokine and cytokine activities,NF-κB signaling pathway,as well as lipid and atherosclerosis.
asthmaulcerative colitisbioinformaticshomotherapy for heteropathycore genetranscription factormolecular mechanism
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