氮杂尿苷对三株腐败菌抑菌活性的测定
Determination of antibacterial activity of 6-azauridine against three strains of spoilage bacteria
刘宫慈 1李锟 1付丹宁 1李乐乐1
作者信息
- 1. 黄河科技学院医学院,郑州 450063
- 折叠
摘要
目的:以二酮哌嗪为母核,对其结构修饰得到的30种化合物进行抑菌活性筛选,找到对大肠杆菌(E.coli)、腐败希瓦氏菌(Shewanella spp.)和铜绿假单胞菌(P.aeruginosa)抑菌效果理想的化合物.方法:首先采用紫外吸收光谱法定期测定放入光照振荡培养箱中菌悬液和营养肉汤的吸光度,得到三株腐败菌的生长曲线.其次采用纸片琼脂扩散法对药物的抑菌活性进行筛选,从30种化合物中筛出有抑菌效果的化合物.最后采用肉汤稀释法结合紫外分光光度计测定最低抑菌浓度(Minimum Inhibitory Concentration,MIC).结果:氮杂尿苷对三株腐败菌的MIC均为3.42 mg/mL,且抑菌效果随着药物浓度的增大而增大.结论:氮杂尿苷对三种腐败菌具有较为显著的抑菌作用,这为后期防腐保鲜剂的研发提供了一定数据参考和理论支持.
Abstract
Objective:30 compounds modified with diketopiperazine as parent core were screened for their antibacterial activities,and the compounds with ideal antibacterial effects against E.coli,Shewanella spp.,and P.aeruginosa were found.Methods:First,the absorbance of bacterial suspension and nutrient broth in the light oscillating incubator was measured by ultraviolet absorption spectrometry,and the growth curves of three putrefying strains were obtained.Secondly,the paper AGAR diffusion method was used to screen the antibacterial activity of the drugs,and the compounds with antibacterial effect were screened out from 30 compounds.Finally,the broth dilution method combined with ultraviolet spectrophotometer was used to determine the Minimum Inhibitory Concentration(MIC).Results:The MIC of 6-azauridine against the three putrefaction strains was 3.42 mg/mL,and the antibacterial effect increased with the increase of drug concentration.Conclusion:6-azauridine has a significant antibacterial effect on the three spoilage bacteria,which provides a certain data reference and theoretical support for the research and development of preservative preservatives in the later stage.
关键词
氮杂尿苷/纸片琼脂扩散法/腐败菌/最低抑菌浓度/抑菌活性Key words
6-azauridine/paper agar diffusion method/rotting bacteria/minimum inhibitory concentration(MIC)/antibacterial activity引用本文复制引用
基金项目
河南省科技攻关项目(222102310388)
出版年
2024
NETL
NSTL
万方数据