Determination of Catechol by a Laccase Biosensor Based on Carbon Dots
Catechol (CC) is one of phenolic compounds,and has been widely used in tanning,cosmetics,flavoring agents,pesticides,dye,medicines and photography chemicals.CC is considered as environmental pollutants by the US Environmental Protection Agency and the European Union due to its high toxicity and low degradability in the ecological system.So far,various approaches have been exploited to meet the rising demands for the determination of CC,including gas chromatography/mass spectrometry,chemiluminescence,synchronous fluorescence,liquid chromatography/ultraviolet spectrometry and pH based-flow injection analysis.Compared with traditional chromatography or spectrophotometry methods,electrochemical methods are preferable and attractive for the detection of CC owing to the advantages of low cost,fast response,excellent selectivity and high sensitivity.Thus in this work,a novel nanomaterial,carbon dots were obtained using phosphorus pentoxide.Then combined with chitosan,a laccase electrochemical biosensor was developed on the surface of glassy carbon matrix modified carbon dots.The electrochemical behaviors of catechol on this biosensor were researched.Experimental results showed that the biosensor showed excellent catalytic activity to the reduction of catechol.In the optimal experimental conditions,the catechol ranged from 1.00× 10-7 to 8.00× 10-5 mol · L-1 had good linear relationship (R=0.999 3) with the detection limit of 7.35 × 10-8 mol · L-1 (S/N =3) with its reduction peak current.The proposed biosensor showed good reproducibility,selectivity and stability,as well as the lower detection limit,high sensitivity and good accuracy,which had potential applications.
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