Effects of circRNA MYLK Gene Interference on Mitochondrial Membrane Potential,Oxidative Damage and Formation of Microtubules in Pancreatic Cancer PANC-1 Cells
Objective To explore the effects of circRNA myosin light chain kinase(MYLK)gene interference on mitochondrial membrane potential,oxidative damage and formation of microtubules in pancreatic cancer PANC-1 cells.Methods PANC-1 cells in logarithmic growth phase were divided into blank control group,shRNA-NC group and circ-MYLK-shRNA group.After transfection,the growth of PANC-1 cells in each group was detected by clone formation as-say.The levels of superoxide dismutase(SOD)and malondialdehyde(MDA)in supernatant of each group were detected by reagent.Mitochondrial membrane potential in each group was detected by flow cytometry.The number of microtubule nodules in each group was observed under the microscope.The expressions of Bcl-2 associated X protein/B-cell lymphoma 2(Bax/Bcl-2),proto-oncogene c-Myc,vascular endothelial growth factor(VEGF)and Vimentin in each group was detected by Western blot.Results Compared with shRNA-NC group,clonal formation rate,percentage of JC-1 red fluo-rescence and number of microtubule nodules of PANC-1 cells in circMYLK-shRNA1 group significantly decreased(all P＜0.05);SOD level in cell supernatant significantly decreased,MDA level significantly increased(all P＜0.05);expres-sion of Bax/Bcl-2 protein in cells significantly increased(P＜0.05),and the protein expressions of c-Myc,VEGF and Vi-mentin significantly decreased(all P＜0.05).Conclusion Silencing circRNA MYLK gene can inhibit the proliferation of PANC-1 cells,reduce mitochondrial membrane potential of PANC-1 cells,induce oxidative damage and inhibit formation of microtubules.
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