Effects of Growth Differentiation Factor 11 on Vascular Repair After Femoral Artery Interventional Injury in Mice
Objective To investigate the effects of growth differentiation factor 11(GDF11)on vascular repair in mice after femoral artery interventional injury.Methods The femoral artery injury model of the C57BL/6 mice was es-tablished by the methods of guide wire damage,30 mice were divided into control group,model group and recombinant human GDF11 protein treatment group(GDF11 group),with 10 mice in each.After 14 days of intervention,the intimal hyperplasia of femoral artery in each group was detected by hematoxylin-eosin(HE)staining,the expression of CD31 in the injured vascular segment of mice in each group was detected by immunofluorescence,the content changes of vascular endothelial growth factor(VEGF),epidermal growth factor(EGF),stromal-derived factor-1(SDF-1)and basic fibro-blast growth factor(bFGF)were detected by enzyme-linked immunosorbent assay(ELISA).Results Compared with control group,the content of vascular repair factors VEGF,SDF-1,bFGF and EGF in peripheral blood increased in mod-el group(all P＜0.01);the area ratio of proliferative intima to media of injured vessels significantly increased(P＜0.01);the expression of CD31 in the model group significantly decreased(P＜0.01).Compared with the model group,the contents of vascular repair factors including VEGF,SDF-1,bFGF and EGF in the GDF11 group significantly in-creased(all P＜0.01);the area ratio of hyperplasia intima to media in the injured segment decreased(P＜0.01);the ex-pression of CD31 significantly increased(P＜0.01).Conclusion GDF11 can inhibit neointimal hyperplasia and promote reen-dothelialization of injured vessels in mice with femoral artery injury,thus playing a role in the repair of injured vessels.
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