Inhibition of Propranolol on Burn-induced Scar in Rats by Negatively-regulating VEGF and PI3K/Akt Signal Pathway
Objective To investigate the effects of propranolol on burn-induced scar in rats and its mechanism.Methods A total of 30 SD rats were randomly divided into control group,model group,low,medium and high-dose pro-pranolol groups,with 6 rats in each.The control group was raised normally;the model groups were constructed by scal-ding the back skin of rats in other groups by scald apparatus with constant temperature and isopiestic pressure.The low-dose,medium-dose and high-dose propranolol groups were treated with 12.5 mg/kg,25 mg/kg and 50 mg/kg propranolol by gavage for 3 weeks respectively.The state and scar status of the rats were observed and recorded every week.The ex-pression of vascular markers CD31 and CD34 in scar cells of each group were detected by immunofluorescence.The mR-NA expressions of vascular endothelial growth factor(VEGF),phosphatidylinositol 3-kinase(PI3K)and protein kinase B(Akt)of scar tissues in each group of rats were detected by reverse transcription quantitative polymerase chain reaction(RT-qPCR);the expressions of VEGF,phos-phorylated protein kinase B(p-Akt)and phosphorylated phosphatidylinositol 3-kinase(p-PI3K)were detected by immunohistochemical method.Results After the treat-ments,compared with the model group,the thickness,vascular distribution and pliability scores of scar tissues in low-dose,medium-dose and high-dose propranolol groups significantly decreased(all P＜0.05).Compared with the model group,the expressions of CD31 and CD34 in scar tissues decreased in low-dose,medium-dose and high-dose propranolol groups(all P＜0.05).Compared with control group,and the mRNA expressions of VEGF,PI3K and Akt,and the pro-tein expressions of p-PI3K,p-Akt and VEGF in scar tissues of rats in model group and low-dose,medium-dose and high-dose propranolol groups significantly increased(all P＜0.01);compared with the model group,the mRNA expressions of VEGF,PI3K and Akt,and the protein expressions of p-PI3K,p-Akt and VEGF in scar tissues of rats in low-dose,medi-um-dose and high-dose propranolol groups were significantly down-regulated(all P＜0.05);compared with the low-dose propranolol group,the mRNA expressions of VEGF,PI3K and Akt,and the protein expressions of p-PI3K,p-Akt and VEGF in scar tissues of rats in medium-dose and high-dose propranolol groups were significantly down-regulated(all P＜0.05);compared with the medium-dose propranolol group,the mRNA expressions of VEGF,PI3K and Akt,and the protein expressions of p-PI3K,p-Akt and VEGF in scar tissues of rats in high-dose propranolol group were significantly down-regulated(all P＜0.05).Conclusion Propranolol inhibits angiogenesis by negatively regulating VEGF and PI3K/Akt signaling pathways,which can effectively treating scars.
ScarPropranololPhosphatidylinositol 3-kinase/protein kinase B signaling pathwayAngiogenesis
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