Observation on the Effects of Tanshinone ⅡA on Hypertrophic Scars in Rabbit Ears and Its Impact on the HIF-1α Signaling Pathway
Objective lo explore the efficacy and possible mechanism of tanshinone ⅡA in the treatment of hyper-trophic scars,and to provide reference for clinical medication in scar treatment.Methods A total of 114 hypertrophic scar models of the rabbit ears were constructed on 12 male and healthy white rabbits,which were randomly divided into control group,triamcinolone acetonide group and tanshinone group,with 38 hypertrophic scars in each group.From 28 days after modeling,the control group was injected with 0.2 ml of saline,the tanshinone group was injected with 0.2 ml of sodium tanshinone ⅡA sulfonate injection,and the triamcinolone acetonide group was injected with 0.2 ml of triamcin-olone acetonide injection,once every 7 days for a total of 4 injections.Scar scale scores and Masson staining were per-formed on the 28th and 56th days after modeling respectively.Samples were taken 56 days after modeling to detect the re-lated indicators.The relative expression levels of hypoxia inducible factor 1 alpha(HIF-1α),transforming growth factor beta 1(TGF-β1)and vascular endothelial growth factor(VEGF)gene were detected by quantitative real time-polymerase chain reaction(qPCR),and the protein expression levels of HIF-1α,TGF-β1 and VEGF were detected by Western blot.Results At 28th day after surgery,there was no significant difference in the score of rabbit ear scar scale among all groups(P＞0.05);at the 56th day after surgery,the decrease in scar scale scores in the tanshinone group and triamcinolone acetonide group was more significant than that in the control group,and the difference was statisti-cally significant(all P＜0.05).At the 28th day of model-ing,Masson staining sections of each group of scar tissues showed collagen fiber blue staining under an upright fluores-cence microscope,the upper part of the dermis was filled with a large number of dense collagen bundles,arranged in a curly and irregular manner,all consistent with the appearance of hypertrophic scars;at the 56th day,Masson staining showed a decrease in collagen fiber accumulation in all groups compared to the 28th day,with a more regular arrange-ment;compared with the control group,the tanshinone group and triamcinolone acetonide group had thinner collagen bundles in the dermis,and there were small blood vessels between the collagen bundles,the arrangement of collagen bun-dles was more regular,and some collagen fibers showed a basket like arrangement similar to normal collagen arrangement in the dermis.Compared with the control group,the genes and protein expressions of HIF-1α,TGF-β1 and VEGF in scar tissues of rabbit ears decreased in the tanshinone group and the triamcinolone acetonide group,and the differences were statistically significant(all P＜0.05);compared with the triamcinolone acetonide group,the HIF-1α,TGF-β1 and VEGF genes and the protein expressions of HIF-1α and VEGF in scar tissues of rabbit ears decreased in the tanshinone group,and the differences were statistically significant(all P＜0.05).Conclusion Tanshinone ⅡA can inhibit the formation of hypertrophic scars in rabbit ears by inhibiting the activation of the HIF-1α signaling pathway.
NETL
NSTL
万方数据
