CTNNB1、TP53蛋白在儿童肝母细胞瘤组织中的表达及与病理特征、预后的关系
Expression of CTNNB1 and TP53 Protein in Hepatoblastoma Tissues of Children and Its Relationship with Pathological Fea-tures and Prognosis
许彩霞 1盛玉1
作者信息
- 1. 210008 江苏南京,南京医科大学附属儿童医院普外科
- 折叠
摘要
目的 探讨钙黏蛋白相关蛋白(cadherin associated protein beta 1,CTNNB1)、肿瘤抑制因子P53(tumor suppressor factor P53,TP53)蛋白在儿童肝母细胞瘤(hepatoblastoma,HB)组织中的表达及与病理特征、预后的关系.方法 选取2017-06/2020-06月作者医院收治的72例HB患儿为研究对象,手术留取癌组织标本及对应的癌旁组织.连续随访3年,记录患儿的预后生存情况,并计算总生存率(overall survival,OS).采用免疫组织化学法检测CTNNB1、TP53蛋白在HB患儿癌组织及癌旁组织中的表达情况;采用x2检验分析CTNNB1、TP53蛋白表达与HB患儿临床病理特征的关系;采用多因素Cox回归分析探讨HB患儿预后的影响因素.结果 HB患儿癌组织中CTNNB1阳性表达率(76.39％)高于对照组(43.06％),TP53阴性表达率(70.83％)高于对照组(38.89％)(P均＜0.05).初诊甲胎蛋白浓度≥ 100 μg/L、POST-TEXT分期Ⅲ～Ⅳ期、肿瘤直径≥3 cm、有肿瘤侵袭或转移HB患儿的CTNNB1阳性表达率、TP53阴性表达率高于初诊甲胎蛋白＜100 μg/L、POST-TEXT分期Ⅰ～Ⅱ期、肿瘤直径＜3 cm、无肿瘤侵袭或转移HB患儿(P均＜0.05).初诊甲胎蛋白≥100 μg/L、POST-TEXT分期Ⅲ～Ⅳ期、有肿瘤侵袭或转移、CTNNB1阳性表达、TP53阴性表达的HB患儿3年OS低于初诊甲胎蛋白＜100 μg/L、POST-TEXT分期Ⅰ～Ⅱ期、无肿瘤侵袭或转移、CT-NNB1阴性表达、TP53阳性表达的HB患儿(P均＜0.05).多因素Cox回归分析结果显示,POST-TEXT分期Ⅲ～Ⅳ期(HR=2.077,95％CI:1.423～3.032)、有肿瘤侵袭或转移(HR=2.291,95％CI:1.536～3.417)、CTNNB1 阳性表达(HR=2.757,95％CI:1.781～4.268)、TP53 阴性表达(HR=2.477,95％CI:1.635～3.753)是 HB 患儿预后的独立危险因素(P＜0.05).结论 HB患儿癌组织中CTNNB1主要呈阳性表达,而TP53主要呈阴性表达,二者与初诊甲胎蛋白、POST-TEXT分期、肿瘤直径、有肿瘤侵袭或转移密切相关,有望作为评估HB患儿预后的生物学指标.
Abstract
Objective To explore the expression of cadherin associated protein beta 1(CTNNB1)and tumor sup-pressor factor P53(TP53)protein in hepatoblastoma(HB)tissues of children and its relationship with pathological fea-tures and prognosis.Methods A total of 72 children with HB admitted to author's hospital from June 2017 to June 2020 were selected as the objects of this study,cancer tissues specimens and corresponding para-carcinoma tissue were retained during the surgery.The patients were followed up for 3 years,and the prognosis was recorded and the overall survival(OS)was calculated.The expressions of CTNNB1 and TP53 protein in cancer tissues and para-carcinoma tissue of HB children were detected by immunohistochemical method;the relationship between the expressions of CTNNB1,TP53 pro-tein and the clinicopathological characteristics of HB children was analyzed by x2 test;the prognostic factors of HB chil-dren were investigated by multivariate Cox regression analysis.Results The positive expression rate of CTNNB1 in the cancer tissues of HB children(76.39％)was higher than that in control group(43.06％),and the negative expression rate of TP53(70.83％)was higher than that in control group(38.89％)(all P＜0.05).The positive expression rate of CTNNB1 and negative expression rate of TP53 in newly diagnosed HB children with alpha-fetoprotein ≥ 100 μg/L,POST-TEXT stage Ⅲ to Ⅳ,tumor diameter ≥3 cm,tumor with invasion or metastasis were higher than those in newly diagnosed HB children with alpha-fetoprotein＜100 μg/L,POST-TEXT stage Ⅰ to Ⅱ,tumor diameter＜3 cm and tumor without invasion or metastasis(all P＜0.05).T he 3-year OS rate of children with alpha-fetoprotein 100μg/L,POST-TEXT stage Ⅲ to Ⅳ,tumor HB with inva-sion or metastasis,positive expression of CTNNB1,nega-tive expression of TP53 was lower than that of newly di-agnosed HB children with alpha-fetoprotein＜100 μg/L,POST-TEXT stage Ⅰ to Ⅱ,tumor without invasion or metas-tasis,negative expression of CTNNB1 and positive expression of TP53(all P＜0.05).Multivariate Cox regression analy-sis showed that POST-TEXT stage Ⅲ～Ⅳ(HR=2.077,95％CI:1.423-3.032),tumor with invasion or metastasis(HR=2.291,95％CI:1.536-3.417),positive expression of CTNNB1(HR=2.757,95％CI:1.781-4.268)and neg-ative expression of TP53(HR=2.477,95％CI:1.635-3.753)were independent risk factors for prognosis of children with HB(P＜0.05).Conclusion The expression of CTNNB1 in the cancer tissues of HB children is mainly positive,while TP53 is mainly negative,the two are closely related to the newly diagnosed alpha-fetoprotein,POST-TEXT stage,tumor diameter and tumor with invasion or metastasis,which are expected to be used as biological indicators to evaluate the prognosis of HB children.
关键词
钙黏蛋白相关蛋白/肿瘤抑制因子P53/儿童/肝母细胞瘤/病理特征/预后Key words
Cadherin associated protein beta 1/Tumor suppressor factor P53/Children/Hepatoblastoma/Patho-logical feature/Prognosis引用本文复制引用
出版年
2024
NETL
NSTL
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