Study of Protective Effect of Recombinant Human Interleukin 35 on Acute Lung Injury Induced by Lipopolysaccharide in Rats
Objective To investigate the protective effect of early intervention of recombinant human interleukin(IL-35)on lipopolysaccharide(LPS)induced acute lung injury(ALI)in rats.Methods A total of 40 male SD rats were randomly divided into normal group,injury group,control group and IL-35 treatment group by random number table method,with 10 rats in each(5 rats at each observation point).The normal group was not treated,while the other 3 groups of rats were given 10 mg/kg LPS through exposed trachea after anesthesia to establish ALI model.After model-ing,the rats in treatment group were injected with 2 μg of recombinant human IL-35 through the tail vein once a day for 3 days;the control group was injected with the same dose of sterile saline via tail vein once a day for 3 consecutive days;the normal group and injury group did not inject any drugs.The pathological changes and scores of lung tissue,arterial partial pressure of oxygen(PaO2)and partial pressure of carbon dioxide(PCO2)of rats in each group were observed on the 1st and 3rd day after treatment.To de-tect content of tumor necrosis factor alpha(TNF-α)and interleukin 10(IL-10)in lung tissues of rats by enzyme-linked immunosorbent assay.Results Compared with normal group,the pathological scores of lung tissue in in-jury group,control group and treatment group were sig-nificantly increased and PaO2 was significantly decreased on the 1st day after modeling(all P＜0.05);the patho-logical score of lung tissue in treatment group was signifi-cantly lower than that in injury group and control group,and the PaO2 was significantly higher than that in injury group and control group,and the PCO2 was significantly higher than that in normal group on the 3rd day(all P＜0.05).Compared with normal group,the contents of TNF-α and IL-10 in injury group,control group and treatment group were significantly increased on 1st and 3rd days(all P＜0.05);the TNF-α content in the treatment group was significantly lower than that in the control group on the 1st and 3rd days(all P＜0.05),the IL-10 content was significantly lower than that in the injury group and the control group on the 1st day,but the IL-10 content was significantly higher than that in the injury group and the control group on the 3rd day(all P＜0.05).Conclusion Recombinant human IL-35 can reduce LPS-induced lung injury,reducing the level of TNF-α in lung injury tissue and increasing the level of IL-10.
Acute lung injuryLipopolysaccharideRecombinant human interleukin-35Cytokines
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