首页|鼻病研究细胞培养实验室"1+2"教学培训模式的效果分析

鼻病研究细胞培养实验室"1+2"教学培训模式的效果分析

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目的 探讨鼻病研究细胞培养实验室"1+2"教学培训模式在实践应用上的效果分析.方法 选取2020~2023年4年间进入鼻病细胞培养实验室的研究生为研究对象,将2020~2021年进入鼻病细胞培养实验室的研究生33人纳入对照组,2022~2023年进入鼻病细胞培养实验室的研究生33人纳入实验组.对照组研究生采用常规实验室老师培训模式;实验组研究生采用"1+2"教学培训模式,即在线上培训交流基础上,结合实践培训模式(导师制模式+实验室制模式).比较两组研究生理论知识考核、实验常规操作行为评估、实践操作考核及培训效果调查的差异.结果 实验组研究生的理论知识考核成绩、实践操作考核成绩明显高于对照组(P均<0.001),且实验组研究生的细胞复苏、细胞传代、细胞冻存、仪器设备正确使用、实验室使用流程、实验器材的消毒灭菌、实验过程中的无菌操作得分均高于对照组(P均<0.05).实验组研究生8项实验常规操作错误行为较对照组均明显减少(P均<0.05).实验组研究生在实验室安全培训效果、实验室考核制度效果、培训后解决实践问题的能力、培训对提升综合能力的作用、整体满意度方面评分均高于对照组(P均<0.05).结论 "1+2"教学培训模式,可提升鼻病研究细胞培养实验室研究生的理论知识、细胞培养技术熟练度和整体实践能力,可促进鼻科学研究中细胞培养实验室的教学质量提升.
Analysis of the Teaching Effectiveness of the"1+2"Training Mode in the Nasal Disease Research Cell Culture Laboratory
Objective To investigate the impact of"1+2"teaching and training mode in the cell culture laboratory of rhinopathy research in practice.Methods The postgraduate students who entered the nasal disease cell culture labora-tory during the four years from 2020 to 2023 were selected as the research objects,33 postgraduate students who entered the laboratory from 2020 to 2021 were included in the control group,while 33 graduate students who joined between 2022 and 2023 were included in the experimental group.The control group adopted the laboratory teacher training mode;whereas the experimental group utilized a"1+2"teaching training mode,that is,on the basis of online training exchange combined with practical training mode(tutorial mode+laboratory mode).The differences of theoretical knowledge test,evaluation of routine experimental operation behavior,practical operation assessment,and investigation of training effec-tiveness were compared between the two groups.Results The test scores of knowledge examination and practical opera-tion of the experimental group were significantly higher than those of the control group(all P<0.001),and the scores of cell resuscitation,cell passage,cell freezing,correct use of instruments and equipment,laboratory use process,disinfec-tion and sterilization of experimental equipment,and aseptic operation in the experimental group were all higher than those in the control group(all P<0.05).The error behavior of routine operation in 8 experiments of the experimental group was significantly reduced compared with that of the control group(all P<0.05).The scores of graduate students in the experimental group were higher than those in the control group in terms of the effect of laboratory safety training,the effect of laboratory assessment system,the ability to solve practical problems after training,the effect of training on improving comprehensive ability and the over-all satisfaction(all P<0.05).Conclusion The"1+2"teaching and training mode can improve the theoretical knowledge,proficiency in cell culture technology,and o-verall practical abilities of the graduate students in the cell culture laboratory of rhinopathy research,consequently,and can promote the teaching quality of scientific research con-ducted in nasal cell culture laboratories.

Nasal disease researchCell culture laboratoryPractical proceduresTheoretical understandingTeaching and training

王平、王向东

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100073 北京,首都医科大学附属北京同仁医院耳鼻咽喉科研究所(北京市耳鼻咽喉科研究所)

鼻病研究 细胞培养实验室 实践操作 理论知识 教学培训

北京市属医学科研院所公益发展改革试点项目北京市自然科学基金项目

京医研2023-17222024

2024

华南国防医学杂志
广州军区医学科学技术委员会

华南国防医学杂志

CSTPCD
影响因子:0.748
ISSN:1009-2595
年,卷(期):2024.38(9)