Objective To explore the application effect of integrated case desktop deduction combined with practical operation mode in the training of nursing staff.Methods A total of 363 nurses working in 24 clinical departments in a grade Ⅲ were selected as the study objects.The department as a unit to carry out integrated case desktop deduction combined with practical operation mode and assessment,the changes of core competence,self-efficacy and job satisfaction of nursing staff before and after intervention were compared.Results After intervention,the scores of core competence,self-efficacy and job satisfaction were significantly increased than those before intervention(all P<0.05).Conclusion The integrated case desktop deduction combined with practical operation mode plays an active role in the training of nursing staff,and it can effectively improve the core competence,self-efficacy and job satisfaction of nursing staff.