热休克蛋白70在儿童噬血细胞性淋巴组织细胞增生症血清中的表达及其临床意义
Expression and Clinical Significance of Heat Shock Protein 70 in Serum of Hemophagocytic Lymphohistiocytosis in Children
卢文婕 1王卓 2杨李 1龙飞 1陈晶佩 1孙鸣 1王伟 1张兰男 2许秀文 2熊昊2
作者信息
- 1. 430010 湖北武汉,华中科技大学同济医学院附属武汉儿童医院儿童血液科
- 2. 430010 湖北武汉,华中科技大学同济医学院血液科
- 折叠
摘要
目的 探究热休克蛋白70(heat shock protein 70,HSP70)在儿童噬血细胞性淋巴组织细胞增生症(he-mophagocytic lymphohistiocytosis,HLH)血清中的表达情况及临床意义.方法 以2019-09/2021-08月作者医院诊断的HLH患儿为HLH组(n=12),脓毒血症患儿为脓毒血症组(n=45),健康查体儿童为对照组(n=32),首次治疗后有缓解的HLH患儿为缓解组(n=11),检测并分析各组儿童血清中HSP70的表达,同时对白细胞介素6(interleukin 6,IL-6)、白细胞介素10(interleukin 10,IL-10)、γ干扰素(interferon gamma,IFN-γ)、淋巴细胞亚群等指标进行检测并分析.结果 HLH组患儿HSP70水平高于脓毒血症组和对照组,差异具有统计学意义(P＜0.001).HLH组患儿IL-10和IFN-γ水平高于脓毒血症组和对照组,差异具有统计学意义(P＜0.001).HLH组患儿IL-6水平高于对照组(P＜0.001),虽低于脓毒血症组,但差异无统计学意义(P＞0.05).HLH组患儿HSP70水平高于缓解组,差异具有统计学意义(P＜0.001).结论 儿童HLH急性期血清中HSP70浓度高于脓毒血症患儿和健康患儿,对早期识别并鉴别诊断儿童HLH和脓毒血症具有潜在临床价值.联合分析HSP70和IL-10、IFN-γ结果有助于早期辅助判断HLH患儿的病情发展趋势和严重程度.
Abstract
Objective To explore the expression and clinical significance of heat shock protein 70(HSP70)in ser-um of hemophagocytic lymphohistiocytosis(HLH)in children.Methods Children who diagnosed with HLH at author's hospital from September 2019 to August 2021 were selected as the HLH group(n=12),Children with sepsis were se-lected as the sepsis group(n=45),while healthy children underwent routine physical examinations were selected as the control group(n=32).Additionally,HLH child patients who achieved remission following initial treatment were classi-fied as the remission group(n=11).The expression of HSP70 in serum of children in each group was detected,the levels of interleukin 6(IL-6),interleukin 10(IL-10),interferon gamma(IFN-γ)and lymphocyte subpopulation were assessed.Results The level of HSP70 in the HLH group was higher than that in sepsis group and control group(P＜0.001).The levels of IL-10 and IFN-γ in HLH group were higher than those in sepsis group and control group(P＜0.001).The IL-6 level was higher in the HLH group compared with control group(P＜0.001),but lower than that in the sepsis group while there was no statistical significance between the two groups(P＞0.05).The serum concentration of HSP70 in the HLH group was higher than that in the remission group(P＜0.001).Conclusion The serum concentration of HSP70 is higher in children with HLH compared with children with sepsis and health children,it suggests that HSP70 can serve as a potential novel biomarker for the early identification and differential diagnosis of HLH and sepsis in child patients.The combination analysis of HSP70,IL-10,and IFN-γoutcomes may facilitate the early evaluation of disease progression and severity in child patients with infectious symptoms.
关键词
噬血细胞性淋巴组织细胞增生症/脓毒血症/热休克蛋白70/白细胞介素10/γ干扰素Key words
Hemophagocytic lymphohistiocytosis/Spesis/Heat shock protein 70/Interleukin 10/Interferon-gamma引用本文复制引用
出版年
2024
NETL
NSTL
万方数据