Using urtica latifolia as raw material and ethanol as extractant,the extraction conditions and in vitro activity of total flavonoids from urtica latifolia are studied by ultrasonic extraction.The effects of ul-trasonic time,ethanol concentration,solid-liquid ratio and ultrasonic times on the extraction of total fla-vonoids from urtica latifolia are investigated by single factor experiment,and the extraction process is op-timized by orthogonal experiment.The results show that the optimum extraction process of total flavonoids in Urtica latifolia is 70%ethanol at a solid-liquid ratio of 1∶50,ultrasonic 20 min,ultrasonic 2 times,the average extraction rate is 12.21%.The total flavonoids of 0.6 g/L has strong scavenging activity on DPPH·free radical and ABTS+free radical,and 1.5 g/L total flavonoids also has certain inhibitory effect on α-amylase and α-glucosidase,which has certain development value.
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