Study on Tissue Culture and Rapid Propagation System of Rose Varieties with Low Survival Rate in Cuttings
Tissue culture and rapid propagation of two rose varieties with low survival rate of cuttings were studied.The lateral buds of double happiness and Golden Mary with strong and full branches and no germination were taken as explants.By comparing germination rate and rooting rate,the most suitable starter medium and rooting medium were selected.The results showed that the optimal starter medium was MS+3 mg/L 6-BA+0.2 mg/L NAA,and the germination rate was 85.7%.The optimal starting medium was MS+2 mg/L 6-BA+0.2 mg/L NAA,and the germination rate was 83.33%.The optimal rooting medium was 1/2 MS+0.5 mg/L IBA+0.2 mg/L NAA.The optimal rooting medium for Golden Mary was 1/2 MS+0.5 mg/L IBA+0.1 mg/L NAA.
Survival rate of cuttingRoseTissue culturegerminationRooting