Karyotype Characteristics and rDNA Distribution Analysis of Cercis glabra Pampan.Chromosomes
We performed the karyotypeanalysis and location of rDNA for Cercis glabra Pampan.chromosomes based on probes of 45S and 5S rDNA repeat sequences and the fluorescence in situ hybridization(FISH)technology.The results of chromosome morphology observation showed that the Cercis glabra Pampan.genome contains three pairs accompanying chromosomes,the chromosome karyotype was 2n=2x=14=14m,which belonged to 1A karyotype with 51.10%of the AS.K(asymmetrical karyotype coefficient).The chromosome length ranging from 2.37 to 4.23 μm,where most chromosomes are of medium size(M1 and M2 types),and the length composition formula is 2L+4M2+8M1.In addition,the FISH results of rDNA indicated that 3 pairs of chromosomes,including the 1th,2th,and 3th pairs,carried 45S rDNAs,2 pairs and 1 pair fluorescence signals were distributed at the short arms end of the chromosome and the middle end of the long arm of the chromosome,respectively;another pair of chromosomes(the 6th pair)carries 5S rDNA,which distributed at the short arm end of the chromosome,and the signal sites of 45S rDNA and 5S rDNA do not overlap.The results will provide more kye information for genetic diversity and genome structure of Cercis glabra Pampan..
Cercis glabra Pampan.ChromosomeKaryotype analysisFluorescence in situ hybridizationrDNA
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