首页|侵染广东黄秋葵的木尔坦棉花曲叶病毒及伴随卫星DNA的分子特征

侵染广东黄秋葵的木尔坦棉花曲叶病毒及伴随卫星DNA的分子特征

扫码查看
原文链接
  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
  • 维普
从广东省表现黄脉曲叶的黄秋葵病株上分离到病毒分离物Okra06,PCR检测结果显示,该病毒属双生病毒科Geminiviridae菜豆金色花叶病毒属Begomovirus.基因克隆及序列分析结果表明,其基因组仅含A组分(DNA - A),全长为2 737 nt,推导编码6个开放阅读框(Open reading frame,ORF).该组分与木尔坦棉花曲叶病毒(Cotton leaf curl Multan virus,CLCuMV)分离物G6的核苷酸序列相似性最高,为99.7％；二者编码的6个ORF相似性分别为100％、100％、99.6％、99.8％、100％和99.7％.该病毒还伴随有卫星分子β(DNA β),全长为1 346 nt,推导其互补链上编码1个ORF (C1).该分子与伴随CLCuMV分离物G6 DNA β的核昔酸序列相似性也最高(99.5％).DNA β系统进化分析显示,Okra06 DNA β与CLCuMV -[ G6] DNA β、CLCuMV -[ Gx08] DNA β亲缘关系最近,三者形成一个独立分支；进一步与其他CLCuMV DNA β和CLCuV DNA β聚类在一起.这些结果证明,侵染广东黄秋葵的病毒分离物Okra06属于CLCuMV,且该分离物与引起广东朱槿曲叶病的CLCuMV-G6应同属木尔坦棉花曲叶病毒朱槿株系.
Molecular Characterization of Cotton leaf curl Multan virus and the Associated Satellite DNA Infecting Okra in Guangdong
The virus isolate Okra06 was obtained from the diseased okra plants exhibiting yellow vein and leaf curl symptoms in Guangzhou, Guangdong Province. The results of PCR detection indicated that the virus belonged to the genus Begomovirus of the family Geminiviridae. The genome of the virus was cloned and sequenced. The results showed that it had only A component(DNA-A). The complete nucleotide sequence of DNA-A was determined to be 2 737 nucleotides, encoding six potential ORFs. The comparison showed that Okra06 DNA-A had the highest nucleotide sequence identity (99. 7% ) with CLCuMV-[ G6]. Six ORFs shared 100% , 100% , 99. 6% , 99. 8% , 100% and 99. 7% nucleotide sequence identities with CLCuMV - [ G6 ], respectively. The Okra06 was also associated with satellite DNA p molecular. The full-length sequence of DN A p was determined to be 1 346 nucleotides, encoding one potential ORF (Cl). The comparison further showed that Okra06 DNA p had the highest nucleotide sequence identity (99. 5% ) with G6 DNA p. Phylogenetic analysis revealed that DNA p of isolate Okra06, G6 and Gx08 were more closely related than the other begomoviruses associated with DNA p, and were clusteredin a separate branch and further clustered with other isolates of CLCuMV DNA 3 or CLCuV DNA f$. Those results revealed that the virus isolate Okra06 infecting okra plants in Guangdong belonged to the species CLCuMV, and it should be the Hibiscus strain.

Okra yellow vein leaf curl diseaseCotton leaf curl Multan virusDNA-Asatellite DNA 3

董迪、朱艳华、何自福、柴兆祥、余小漫、罗方芳

展开 >

广东省农业科学院植物保护研究所,广东广州510640

甘肃农业大学草业学院,甘肃兰州730070

黄秋葵黄脉曲叶病 木尔坦棉花曲叶病毒 DNA-A 卫星DNA β

国家自然科学基金公益性行业(农业)科研专项广东省自然科学基金广东省国际科技合作项目省部产学研结合项目

3067008520100306591510640010000312007A0501000052008B090500212

2012

华南农业大学学报
华南农业大学

华南农业大学学报

CSTPCDCSCD北大核心
影响因子：0.837
ISSN：1001-411X
年,卷(期)：2012.33(1)
  • 18
  • 9