Cloning and expression analysis of sucrose non-fermenting 1-related protein kinase 2.7(SnRK2.7)gene in Hevea brasiliensis
To explore the role of sucrose non-fermentation associated protein kinase 2(SnRK2)in the response of Hevea brasiliensis to low temperature stress,a member of the SnRK2 subfamily,named HbSnRK2.7,was cloned from the leaves of the cold-resistant clone Reyan'93-114'of Hevea brasiliensis by using RT-PCR.The gene cloned contains an open reading frame of 1 095 bp that encodes a protein of 364 amino acids.HbSnRK2.7 has a conserved serine/threonine protein kinase domain with a molecular weight of 41.39 kD and an isoelectric point of 4.70.It is predicted that it can catalyze the transfer of phosphate groups from ATP to the serine/threonine residues on the protein substrate,which is also the concentrated region of phosphorylation sites.It also has domains needed for abiotic stress and ABA-dependent domains for HbSnRK2.7 activation.Quantitative PCR(qPCR)results showed that HbSnRK2.7 gene was down-regulated in response to ABA induction.When treated with ABA for 8 h,the expression was down-regulated to the lowest point.Under low temperature stress,the expression of HbSnRK2.7 decreased significantly.The expression of HbSnRK2.7 was down-regulated continuously under low temperature stress for 4 h and 8 h,and the expression of HbSnRK2.7 was down-regulated to the lowest level at 24 h under low temperature stress,which was about 1/2 of non-stress expression.Moreover,the expression of HbSnRK2.7 was significantly higher in the cold-susceptible clones than in the cold-resistant clones.These results suggest that HbSnRK2.7 may act as a negative regulatory factor to mediate the formation of low temperature stress resistance of Hevea brasiliensisdependent on ABA.
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维普
