Effects of host identity and leaf traits on foliar endophytic fungal communities in Lauraceae and Fagaceae plants of tropical montane rainforest of Hainan Island
To investigate the effects of plant identity and leaf traits on foliar endophytic fungal community assembly,the species composition of foliar endophytic fungi in dominant plants in the tropical mountain rainforest in Jianfengling,Hainan Island were determined by using the Illumina Miseq sequencing method,based on which the effects of host identity and leaf traits on the species composition of foliar endophytic fungi were explored.A total of 1,539,567 fungal ITS2 sequences were obtained from the leaves of 8 species of Lauraceae plants and 7 species of Fagaceae plants,which were classified into 5,471 Operational Taxonomic Units(OTUs)belonging to 8 phyla,30 classes,108 orders,281 families,and 892 genera.Ascomycota was the largest group of the foliar endophytic fungi,accounting for 94.5%of all the fungal sequences.The number of endophytic fungal species in the leaves of individual Lauraceae plants(805±32)OTUs was significantly higher than that of Fagaceae plants(554±41)OTUs.However,there was no significant difference in Shannon-Wiener and Simpson diversity indices between the endophytic fungi in the leaves of the plants of these two families.Partial redundancy analysis showed that the host plant identities of family,genus and species significantly affected the composition of endophytic fungal communities.After removing the effect of plant traits,the host plant identities of family,genus,and species could independently explain 2.9%,15.7%,and 33.7%of the variation in endophytic fungal species composition,respectively.After removing the effect of host plant identity,leaf traits could explain 21.3%of the variation in endophytic fungal species composition(P =0.003),of which leaf calcium content,specific leaf area,leaf nitrogen content,and leaf potassium content had significant effects on the composition of endophytic fungal communities.These findings suggest that host identity and leaf traits are important factors driving the assembly of endophytic fungal communities in the tropical mountain rainforest.
NETL
NSTL
万方数据
维普
