Identification and functional analysis of candidate effector FoSSP20 in Fusarium oxysporumf.sp.cubense
Fusarium wilt of banana caused by Fusarium oxysporum f.sp.cubense(Foc)is destructive to banana(Musa spp.)industry.Effector proteins play a key role in the interaction between Foc and banana.Previously researches screened the candidate effector FoSSP20 in Foc race 4(Foc4)which could suppress BAX-triggered programmed cell death(PCD).In this study,yeast secretion system was used to verify the secretory function of signal peptide of FoSSP20.Subcellular localization assay showed that the FoSSP20 was localized in the nucleus and plasma membranes in Nicotiana.benthamiana cells.qRT-PCR assay showed that the expression levels of FoSSP20 was significantly up-regulated at the early infection phase.All the results showed that effector FoSSP20 is a classic secretory protein which may function well in the infection process of pathogens.
Fusarium oxysporumf.sp.Cubenseeffectorsubcellular localizationcell death
NETL
NSTL
万方数据
维普
