为研究木薯(Manihot esculenta)内生细菌多样性,挖掘木薯内生细菌资源,采用16S rDNA测序技术对'SC8'和'SC9'2个木薯品种的块根、茎和叶等不同组织进行了 Alpha和Beta多样性分析,分析细菌群落结构组成并对比Kyoto Encyclopedia of Genes and Genomes(KEGG)数据库进行内生细菌菌群基因功能预测.结果显示,从'SC8'木薯样品中共聚类得到19,087个细菌OTUs(operational taxonomic units)隶属于48个门、126个纲、438个科和805个属;'SC9'聚类到20,148个细菌OTUs隶属于46个门、130个纲、390个科和863个属.两品种各组织共有内生菌属171个,占属种类的78.44％,'SC9'特有菌群118个,'SC8'为100个,茎(韧皮部)内生菌多样性最为丰富.2品种内生细菌的优势菌群有变形菌门、蓝细菌门、放线菌门、拟杆菌门、厚壁菌门和酸杆菌门.木薯内生细菌功能中涉及多种萜类、酮类、氨基酸及维生素等次级代谢产物的生物合成.研究结果表明,木薯植物体样品中均含有丰富的内生细菌种类,且在茎(韧皮部)中的多样性最高;木薯含有多种活性成分,这些活性物质的代谢相关通路同样在功能预测中有所体现.
Diversity of entophytic bacteria in different tissues of cassava
In order to study the diversity of endophytic bacteria in cassava(Manihot esculenta)and explore the endophytic bacteria resources,16S rDNA sequencing technology was used to analyze the Alpha and Beta diversity of tuber,stem and leaf tissues in'SC8'and'SC9'cassava varieties.The structural composition of bacterial community was analyzed and the gene function of endophytic bacteria community was predicted by comparing with Kyoto Encyclopedia of Genes and Genomes(KEGG)database.The results showed that 19,087 bacterial OTUs(operational taxonomic unit)were clustered from'SC8'cassava samples,and belonged to 48 phyla,126 classes,438 families and 805 genera,and 20,148 bacterial OTUs belonged to 46 phyla,130 classes,390 families and 863 genera in'SC9'.There were 171 common bacteria genera in all tissues of cassava,accounting for 78.44％of all endophytic bacteria species.There were 118 unique bacteria in'SC9'and 100 in'SC8',and endophytic bacteria in stem(phloem)had the highest diversity.The dominant bacterial groups of endophytic bacteria from cassava are Proteobacteria,Cyanobacteria,Actinobacteria,Bacteroidetes,Firmicutes and Acidobacteria.Biosynthesis of terpenoids,ketones,amino acids,vitamins and other secondary metabolites is involved in the function of cassava endophytic bacteria.These results showed that there were abundant endophytic bacteria in all cassava plant samples,and the diversity was highest in stem(phloem).Cassava contains a variety of active ingredients,and the metabolism-related pathways of these active ingredients are also reflected in the function prediction.
万方数据
维普
