GNAT acetyltransferase regulates the growth and virulence of Xanthomonas oryzae pv.oryzicola
To investigate the function of GNAT acetyltransferases in Xanthomonas oryzae pv.oryzicola(Xoc),a pathogen of rice bacterial leaf streak,we performed domain analysis of GNAT family proteins in Xoc,and then constructed the single and multiple mutants of these GNAT genes.Finally,we compared the growth rates,extracellular enzyme activities,and virulence of these mutants and the wild type Xoc strains.The results showed that GNAT acetyltransferases regulated Xoc growth in nutrient deficiency media.All mutants exceptΔxoc_1598 and A6 grew more slowly than the wild type,and had lower ability of infection than the wild type strain.We also found that mutation in acetyltransferases could alter motility and the activities of the extracellular protease and amylase.All the results showed that protein acetylation is an important regulatory mechanism for Xoc growth and virulence as well as the virulence-related factors'production.
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