Genome-wide identification and expression analysis of GRF gene family in litchi
In order to reveal the functions of litchi GFR gene,the whole genome of litchi growth-regulating factor(LcGRF)gene family was identified and analyzed,and its expression patterns in different tissues of litchi were studied.Based on the litchi genome database,bioinformatics software was used to conduct genome-wide identification of members of the LcGRFs family,an analysis was made of basic physical and chemical properties,chromosome localization,gene structure,evolutionary relationships,protein conserved motifs,cis-acting elements,and spatiotemporal expression for systematic analysis.A total of 12 GRF genes were obtained from litchi,which were unevenly distributed on 10 chromosomes,with 2-5 introns.The analysis of the conserved motifs of the GRF protein found that all the GRF proteins in litchi contained conservative motifs of motif 1(WRC)and motif 2(QLQ).According to evolutionary analysis,LcGRF was divided into five subfamilies.There were a large number of cis-acting elements related to light,plant hormones,abiotic stress responses and growth and development on the promoter of LcGRFs.The results of different transcriptome expression patterns show that LcGRFs exhibits diverse expression characteristics in various tissues,indicating that different members may play regulatory roles in different growth and development processes of litchi,participating in regulating the growth and development of litchi.The analysis shows that there are 12 members of the litchi GRF family,which are divided into 5 subfamilies.