Whole-genome analysis of the HubHLH gene family in Pitaya and its differential expression in response to supplementary light-induced flowering in winter
In order to obtain relative complete candidate genes,the HubHLH gene expression response in Pitaya during the flowering process induced by supplementary light in winter was investigated,and the whole genome of HubHLH gene family in Pitaya was analyzed.There were 153 pitaya HubHLH genes identified,and their encoding proteins contained 176-687 amino acids with their molecular weight being 19.28-74.44 kDa,pI 4.81-9.88.These proteins were all hydrophilic.The prediction of subcellular localization showed that most of the proteins were localized in the nucleus.The pitaya HubHLH proteins were compared with 120 Arabidopsis AtbHLH proteins for phylogenetic analysis.Phylogenetic analysis showed that the pitaya HubHLH proteins were divided into 12 groups and 25 subfamilies.Analysis of conserved motif,gene structure and location distribution in chromosomes showed that genes of the same subfamily had similar motif composition and gene structure.Analysis of the internal replication events of the pitaya HubHLH gene family found that 78 genes were identified as fragment repeating genes,indicating that fragment replication was the main expansion force of the pitaya HubHLH gene family.In addition,based on the transcriptomic data of the four phases of supplementary light-induced flowering of Pitaya in winter,59 HubHLH genes were differentially expressed during the process of supplementary light induced flowering in winter.GO function enrichment showed that they were enriched in response to red or far-red light,light stimulation,sexual reproduction function,and radiation.The HubHLH gene family may play a regulatory role in the flowering process of Pitaya induced by supplementary light in winter.In this study,we conducted a comprehensive genome analysis of the HubHLH gene family in Pitaya,obtained relatively complete candidate genes,and preliminarily investigated the expression response of HubHLH genes in the winter flowering process induced by supplementary light in Pitava.
万方数据
维普
