首页|盐胁迫下冰菜盐囊泡细胞的比较代谢组学分析

盐胁迫下冰菜盐囊泡细胞的比较代谢组学分析

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为了研究盐胁迫下冰菜(Mesembryanthemum crystallinum)盐囊泡中代谢物的变化,通过液相色谱串联质谱(LC-MS/MS)技术对对照以及盐处理下冰菜的盐囊泡中的代谢物进行了鉴定及分析,共在冰菜盐囊泡中确定了 356个已知代谢物.对差异代谢物的筛选结果表明,有37种代谢物的含量在盐处理后的盐囊泡中发生了显著变化.基于KEGG数据库对差异代谢物进行的代谢通路富集分析表明,KEGG中定义的植物激素信号传导、玉米素生物合成及嘌呤代谢等6条生化途径存在显著扰动.以上结果显示,盐处理能造成冰菜盐囊泡中的代谢物发生显著变化.
Comparative metabolomic analysis of epidermal bladder cells in Mesembryanthemum crystallinum under salt stress
To investigate the changes of metabolites in epidermal bladder cells of the halophytic species Mesembryanthemum crystallinum under salt stress,the metabolites in the epidermal bladder cells of M.crystallinum under control and salt stress were identified and analyzed by using the liquid chromatography tandem mass spectrometry(LC-MS/MS),and a total of 356 known metabolites were identified in the epidermal bladder cells.Analysis of differential metabolites showed that the levels of 37 metabolites in the epidermal bladder cells were significantly changed under the salt stress.Metabolic pathway enrichment analysis of the differential metabolites based on the KEGG database showed significant perturbations in six biochemical pathways defined in KEGG,such as phytohormone signaling,zeatin biosynthesis,purine metabolism,etc.These results indicate that salt treatment can cause significant changes in metabolites in epidermal bladder cells of M.crystallinum.

salt stressMesembryanthemum crystallinumepidermal bladder cellsmetabolome

朱昆宇、潘成才、吉怡颖、王宇、周扬、江行玉

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海南大学热带作物学院/海南省耐盐作物重点实验室,海口 570228

海南大学园艺学院,海口 570228

广东海洋大学滨海农业学院,广东湛江

盐胁迫 冰菜 盐囊泡 代谢组

国家自然科学基金国家自然科学基金海南省自然科学基金

2018YFD10005002018YFE0207203-22019RC046

2024

热带生物学报
海南大学

热带生物学报

CSTPCD
影响因子:0.406
ISSN:1674-7054
年,卷(期):2024.15(2)
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