Isolation of plant protoplast and its application in transient transformation
The cell wall was removed from plant cells,and the cytoplasm wrapped by a single cellmembrane is called protoplast.It has been widely used in protein subcellular localization,gene expression analysis,protein-protein interaction and gene editing among others.The factors influencing plant protoplasts isolation by enzymatic method,the effects of electroporation and PEG-mediated methods on the transient transformation efficiency of protoplasts and their application in transient transformation were summarized in this review.Plant species,different PEG concentrations,transfection time,DNA concentration,and number of protoplasts were found to affect the PEG-mediated transient transformation efficiency of protoplasts.The electroporation had an optimum transformation efficiency of protoplasts only under the adequate conditions of pulse voltage,pulse duration,pulse number,cell number,and DNA concentration.This review might provide reference for establishment of more plant protoplast isolation systems and transient transformation systems for plant protoplasts.
万方数据
维普
