Analysis of aporphine alkaloids in Cassytha filiformis
In order to discover aporphine alkaloids with hypoglycemic activity in Cassytha filiformis,the total alkaloids were extracted from C.filiformis by acid solubilization and alkaline precipitation,obtained by ethyl acetate extraction,separated and purified by various chromatographic methods.The structures of the compounds purified were identified by using spectrometry,and the glucose oxidase method was used to evaluate the glucose consumption activity of the resulting aporphine alkaloids on HL-7702 cells.Thirteen aporphine alkaloids were identified from the extract of C.filiformis:cassamedine(1),cassameridine(2),thalicminine(3),neolitsine(4),N-metilseconeolitsine(5),N-methyl-2,3,6-trimethoxymorphin-andien-7-one(6),1,2-methylenedioxy-3,10,11-trimethoxyaporphine(7),cassythidine(8),norneolitsine(9),O-methylcassythine(10),cassythine(11),filiformine(12),and 1,2-Dimethoxy-5,6,6a,7-tetrahydro-4H-benzo[de][l,3]benzodioxolo[56-g]quinoline(13).Among them,compounds 5 and 13 were first isolated from C.filiformis.Cellular glucose consumption activity assay showed that compounds 3 and 11 significantly promoted glucose consumption in HL-7702 cells and displayed a potential value for research and development into hypoglycemic lead compounds.
万方数据
维普
