摘要
为了寻找无根藤(Cassytha filiformis)中具有降糖活性的阿朴菲类生物碱,以无根藤为研究对象,通过酸溶碱沉法提取,乙酸乙酯萃取获取总生物碱,并通过多种色谱方法进行分离和纯化,再通过波谱学技术鉴定化合物的结构,最后采用葡萄糖氧化酶法评价所得阿朴菲类生物碱对HL-7702细胞葡萄糖消耗活性.鉴定结果发现13个阿朴菲类生物碱,分别鉴定为:无根藤米丁(1)、无根藤米里丁(2)、小唐松草宁碱(3)、新木姜子素(4)、N-metilseconeolitsine(5)、N-methyl-2,3,6-trimethoxymorphin-andien-7-one(6)、10-O-甲基汝兰碱(7)、无根藤定碱(8)、nomeolitsine(9)、O-甲基无根藤碱(10)、无根藤碱(11)、filiformine(12)、降南天竹碱(13),其中,化合物5和13首次从该植物中分离得到.细胞葡萄糖消耗活性结果显示,化合物3和11显著促进HL-7702 细胞葡萄糖消耗,具有研究开发成降糖先导化合物的潜在价值.
Abstract
In order to discover aporphine alkaloids with hypoglycemic activity in Cassytha filiformis,the total alkaloids were extracted from C.filiformis by acid solubilization and alkaline precipitation,obtained by ethyl acetate extraction,separated and purified by various chromatographic methods.The structures of the compounds purified were identified by using spectrometry,and the glucose oxidase method was used to evaluate the glucose consumption activity of the resulting aporphine alkaloids on HL-7702 cells.Thirteen aporphine alkaloids were identified from the extract of C.filiformis:cassamedine(1),cassameridine(2),thalicminine(3),neolitsine(4),N-metilseconeolitsine(5),N-methyl-2,3,6-trimethoxymorphin-andien-7-one(6),1,2-methylenedioxy-3,10,11-trimethoxyaporphine(7),cassythidine(8),norneolitsine(9),O-methylcassythine(10),cassythine(11),filiformine(12),and 1,2-Dimethoxy-5,6,6a,7-tetrahydro-4H-benzo[de][l,3]benzodioxolo[56-g]quinoline(13).Among them,compounds 5 and 13 were first isolated from C.filiformis.Cellular glucose consumption activity assay showed that compounds 3 and 11 significantly promoted glucose consumption in HL-7702 cells and displayed a potential value for research and development into hypoglycemic lead compounds.
基金项目
海南省重点研发计划(ZDYF2022SHFZ037)
海南省自然科学基金面上项目(221MS040)
海南省研究生创新科研项目(Qhys2021-364)
维普
万方数据