Analysis of the chemical composition of the essential oils from floral buds and leaves of Michelia shiluensis and their inhibitory activity against α-glucosidase
Essential oil was extracted from floral buds and leaves of Michelia shiluensis(M.shiluensis)by steam distillation.After treatment,the components of the essential oil were determined by gas chromatography-mass spectrometry(GC-MS).The GC-MS analysis showed that the main components of the two essential oils were sesquiterpenes.A total of 39 chemical components were identified from the essential oil of floral buds,of which three,β-bisabolene,α-curcumene and β-elemene,were the highest in relative content.A total of 31 chemical constituents were identified from the essential oil of leaves,including guaiacol,isoguaiacol,(Z,Z,Z)-1,8,11,14-heptadecatetraene,and so on.The relative content of alc cohols and olefins is an important index to distinguish between the essential oils of the floral buds and the leaves.The components of M.shiluensis essential oil were analyzed for the first time,and the inhibitory activity of M.shiluensis essential oil on α-glucosidase was determined.When the mass concentration was 3 g·L-1,the essential oils of the leaves and floral buds had an inhibition rate of 87.13％and 72.27％against α-glucosidase activity,and their IC50 was 0.73±0.04 g·L-1 and 1.77±0.07 g·L-1,respectively.M.shiluensis essential oil has a strong inhibitory effect on α-glucosidase activity,and can be developed and used as a hypoglycemic efficacy factor,which provides a theoretical basis for value-added utilization of M.shiluensis resources.
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