Identification of Pissodes castaneus(DeGeer),a quarantine pest
Pissodes castaneus is an important invasive forest pest and a phytosanitary pest in China.The insect pest is native to European countries and has been introduced into Africa and South America.There is no record of the distribution of the insect pest in China,and it was first intercepted at Yangpu Port in Hainan,China in August 2017.P.castaneus mainly infests the cambium and phloem of Pinaceae plants and form pupa chambers under the bark,and it can be spread over long distances through imported timber trade.The adults,larva and pupa of an insect were collected at Yangput Port in August 2017,and their mitochondrial COI genes were amplified by using DNA barcoding and compared with those in GenBank database and BOLD database.The phylogenetic tree of this insect was constructed by using the neighbor-joining algorithm.The results showed that the insect sample and P.castaneus were clustered into the same family and had more than 99％homology.This DNA barcoding method provides a scientific basis for rapid quarantine clearance and detection of invasive forest pests.
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