Effects of explant position,section length and culture temperature on the propagation of Betaphycus gelatinae
Betaphycus gelatinae was cut into sections of different lengths from different explants and cultured at different temperatures to explore the suitable conditions for its tissue culture to determine the effects of different explants(stem tip,main branch and trunk),section lengths(5 mm,10 mm and 15 mm)and culture temperatures(21 ℃,24 ℃,27 ℃,30 ℃ and 33 ℃)on bud emergence and growth of the explants of B.gelatinae.The results showed that the survival rate and bud emergence rate of different explants and section lengths were all up to 100％.The number of buds in different explants was in the order of the trunk＞the main branch＞the stem tip.The stem tip mainly grew elongate,while the main branch and trunk were mainly expanded and formed buds.The buds were formed on the upper cut,not on the lower cut,showing obvious polarity.The number of the buds in the sections of different lengths was in the order of the sections 15 mm long＞the sections 10 mm long＞the sections 5 mm long.The number of individual buds in the sections 15 mm long was 5.90±0.67 in the stem tip,10.16±1.14 in the main branch and 11.90±0.88 in the trunk.The highest budding density was in the sections 5 mm long.The suitable growth temperature for tissue culture of B.gelatinae ranged from 21 ℃ to 27 ℃,under which the survival rate and bud emergence rate were both 100％,and the number of buds increased with the temperature.The survival rate and bud emergenc rate of the sections were decreased when the temperature was above 27 ℃.For optimal tissue culture B.gelatinae was cultured at 27 ℃ with the trunk sections 15 mm long as explants.
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