摘要
采用多种分离纯化方法,包括硅胶、凝胶、薄层色谱以及半制备高效液相等;并结合瘤背石磺生化特性、NMR数据分析及文献比较,对化合物进行结构鉴定.从瘤背石磺中分离鉴定出12个化合物,经鉴定结构为Cyclo-(L-Pro-L-Val)(1)、Cyclo-(Pro-Ile)(2)、Cyclo-(L-Pro-D-Leu)(3)、Tyramine(4)、Cyclo-(L-Pro-L-Phe)(5)、2-哌啶酮(6)、Cyclo-(L-Ala-L-Pro)(7)、尿嘧啶核糖核苷(8)、尿嘧啶(9)、胸腺嘧啶(10)、2'-甲氧基尿嘧啶核苷(11)、胸苷(12).化合物(1～6,11)均为首次从瘤背石磺中分离得到.
Abstract
Onchidium verruculatum,a characteristic resource in the South China Sea,was extracted and isolated by using a variety of separation and purification methods,including silica gel,Sephadex LH-20,thin layer chromatography and semi-prepared HPLC.The structures of the compounds were identified by combining their biochemical characteristics,NMR data analysis and reference comparison.Twelve compounds were isolated from Onchidium verruculatum,and their structures were identified as Cyclo-(L-Pro-L-Val)(1),Cyclo-(Pro-Ile)(2),Cyclo-(L-Pro-D-Leu)(3),Tyramine(4),Cyclo-(L-Pro-L-Phe)(5),2-Piperidone(6),Cyclo-(L-Ala-L-Pro)(7),uracil ribonucleoside(8),uracil(9),thymine(10),2'-methoxyuracil nucleoside(11),thymidine(12).Compounds(1-6,11)were isolated for the first time from Onchidium verruculatum.
基金项目
海南省重点研究发展计划(ZDYF2022SHFZ037)
国家自然科学基金(82060778)
NETL
NSTL
万方数据