大豆GmACBP6基因克隆及萌发期耐盐碱功能验证
Cloning of soybean GmACBP6 gene and functional Validation of salinity tolerance during germination
吴管吉 1周勋峻 1吴菲菲 1张引鹤 1黄颖华 1李雅忻 2高红桃 2李海燕2
作者信息
- 1. 海南大学 热带农林学院,海口 570288
- 2. 海南大学 热带农林学院,海口 570288;海南大学 三亚南繁研究院,海南 三亚 572024
- 折叠
摘要
为了明确大豆(Glycine max)酰基辅酶A结合蛋白(GmACBP6)是否具有耐盐碱功能,本研究首先利用荧光定量PCR方法对大豆GmACBP6萌发期盐碱胁迫下的表达量进行分析,明确GmACBP6在大豆萌发期对盐碱胁迫具有响应.在此基础上,构建pYES2-GmACBP6重组载体并转化酵母菌株,观察转pYES2-GmACBP6酵母菌株及转pYES2酵母菌株在盐及盐碱胁迫培养基中的生长情况,结果表明,转入pYES2-GmACBP6酵母菌株在盐及盐碱胁迫长势明显优于转pYES2空载体的酵母菌株,初步确定大豆GmACBP6基因具有提高酵母细胞盐碱耐受性的功能.同时,本研究通过创制GmACBP6回补及超表达拟南芥(Arabidopsis thaliana)材料,并对其萌发期耐盐碱表型进行鉴定,结果表明,在盐及盐碱胁迫下GmACBP6超表达拟南芥种子的萌发率显著高于GmACBP6回补材料、atacbp6突变体及野生型拟南芥,进一步确定了大豆GmACBP6基因具有耐盐碱功能.
Abstract
In order to determine whether soybean(Glycine max)acyl-Coenzyme A binding protein(GmACBP6)has saline-tolerant function,the expression of GmACBP6 during the germination period was analyzed by using the real-time PCR,and analysis showed that GmACBP6 responded to saline-alkali stress in the germination period of soybean.In this context the pYES2-GmACBP6 recombinant vector was constructed and transformed into the yeast strains,The growth of pYES2-GmACBP6 yeast strain and pYES2 yeast strain cultured in salt and saline stress mediums were observed.The results show that the pYES 2-GmACBP6 yeast strain grew significantly better under salt and salt stress than the pYES2 yeast vector.It is preliminarily determined that the soybean GmACBP6 gene has the function of improving the salt and alkali tolerance of yeast cells.In this study the A.thaliana materials with GmACBP6 knock-in and overexpression were also produced,and their salinity-tolerant phenotype during the germination stage was identified.The results showed that the germination rate of Arabidopsis seeds with GmACBP6 overexpression was significantly higher than that of GmACBP6 knock-in,atacbp6 mutant or wild Arabidopsis thaliana under salt and salt-alkali stress,which further confirmed the salt-alkali tolerance of the GmACBP6 gene in the soybean.
关键词
大豆/酰基辅酶A结合蛋白GmACBP6/耐盐碱性Key words
soybean/acyl-CoA binding protein GmACBP6/salt and alkali tolerance引用本文复制引用
基金项目
国家自然科学基金项目(32171793)
海南大学人才引进启动资金项目(Y3AZ20024)
出版年
2024
NETL
NSTL
万方数据