Cloning of soybean GmACBP6 gene and functional Validation of salinity tolerance during germination
In order to determine whether soybean(Glycine max)acyl-Coenzyme A binding protein(GmACBP6)has saline-tolerant function,the expression of GmACBP6 during the germination period was analyzed by using the real-time PCR,and analysis showed that GmACBP6 responded to saline-alkali stress in the germination period of soybean.In this context the pYES2-GmACBP6 recombinant vector was constructed and transformed into the yeast strains,The growth of pYES2-GmACBP6 yeast strain and pYES2 yeast strain cultured in salt and saline stress mediums were observed.The results show that the pYES 2-GmACBP6 yeast strain grew significantly better under salt and salt stress than the pYES2 yeast vector.It is preliminarily determined that the soybean GmACBP6 gene has the function of improving the salt and alkali tolerance of yeast cells.In this study the A.thaliana materials with GmACBP6 knock-in and overexpression were also produced,and their salinity-tolerant phenotype during the germination stage was identified.The results showed that the germination rate of Arabidopsis seeds with GmACBP6 overexpression was significantly higher than that of GmACBP6 knock-in,atacbp6 mutant or wild Arabidopsis thaliana under salt and salt-alkali stress,which further confirmed the salt-alkali tolerance of the GmACBP6 gene in the soybean.
soybeanacyl-CoA binding protein GmACBP6salt and alkali tolerance
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