五指山野生大叶茶叶片代谢物鉴定和特征分析
Identification and characteristic analysis of metabolites from the leaves of wild Assam tea(C.sinensis var.assamica)in Wuzhishan
苟志辉 1杜尚嘉 1符生波 1符明珠 1符溶 1吴海霞1
作者信息
- 1. 海南省林业科学研究院/海南省红树林研究院,海口 571100
- 折叠
摘要
为了解五指山野生大叶茶(Camellia sinensis var.assamica)代谢物的特征,利用代谢组学方法,对采集自五指山地区的野生茶树和人工茶园茶叶的代谢物成分开展了比较研究,在野生茶树茶叶中鉴定得到398个含量显著上调的代谢物和247个显著下调的代谢物.基于偏最小二乘法判别分析表明,野生茶叶和人工茶叶分别聚为一类.富集分析结果显示,野生茶树叶片中含量显著上升的代谢物的功能主要富集于糖酵解、丙酮酸合成、氨基糖代谢和核苷糖代谢等糖代谢通路,以及酚类物质合成、核黄素代谢、鞘磷脂代谢和黄酮合成等通路,而显著下降的代谢物的功能主要富集于氨基酸代谢和油脂代谢等初生代谢.
Abstract
Tea prepared from wild tea leaves in the Wuzhishan region have become popular in the market over recent years.However,its metabolomic characteristics are seldom reported.An attempt was made to compare the metabolites found in the leaves of wild and cultivated tea in Wuzhishan,Hainan,China by employing a metabolomic method.A total of 398 metabolites were identified significantly up-regulated,and 247 were significantly down-regulated in the wild tea leaves compared to those from the leaves of tea cultivated in the tea plantations.Partial least squares discrimination analysis suggested that wild and cultivated tea could each be grouped into their respective categories.Enrichment analysis indicated that the functions of the metabolites with significant high content in the wild tea leaves were mainly concentrated in metabolic pathways such as glycolysis,pyruvate synthesis,amino sugar metabolism,and nucleotide sugar metabolism,as well as pathways for the synthesis of phenolic substances,riboflavin metabolism,sphingolipid metabolism,and flavonoid synthesis.The functions of the metabolites with significantly low content were mainly enriched in primary metabolic pathways,such as amino acid metabolism and lipid metabolism.
关键词
野生茶叶/五指山/代谢组/糖/酚Key words
wild tea/Wuzhishan/metabolomics/carbohydrate/phenol引用本文复制引用
基金项目
海南省省属科研院所技术创新专项(SQKY2022-0037)
海南省省属科研院所技术创新项目(KYYSLK2023-021)
五指山市科技项目(WZSKJXM2021005)
出版年
2024
NETL
NSTL
万方数据