Effects of intercropping of banana with green manure crops on soil nitrogen transformation and banana leaf enzyme activity
An attempt was made to explore the changes of soil nitrogen transformation and nitrogen metabolism in banana plantations intercropped with leguminous green manure crops.A pot experiment was conducted to set up five treatments:monoculture of banana(CK),intercropping with Trifolium repens L(BT),intercropping with Melilotus officinalis(BM),intercropping with Lolium perenne L(BR).The changes of soil nutrient content and key enzymes for nitrogen metabolism in different sampling periods were determined.The results showed that the soil pH and the contents of organic matter,available nitrogen,total nitrogen,available phosphorus and available potassium in banana and intercrops,leguminous green manure crops,were increased in different degrees,and also increased were the soil contents of NH4+-N and NO3--N,and the net mineralization rate and the net nitrification rate of nitrogen in the soil.After intercropping with leguminous green manure crops,the activities of nitrate reductase(NR),nitrite reductase(NiR)and glutamine synthetase(GS)in banana leaves were significantly higher than those in the monoculture banana.The soil NH4+-N and NO3--N were significantly positively correlated with the NiR,GS and glutamate synthetase(GOGAT)in the banana leaves.
leguminous green manurebananaintercroppingnitrogen transformationenzyme activity