Application of DNA barcoding in rapid identification of Contarinia maculipennis Felt infesting Dendrobium nobile
Dendrobium nobile has great medicinal value,unique flower shape and rich color,and hence is well favored in the international market and widely planted in tropical and subtropical areas.The larvae of Contarinia maculipennis Felt are hidden in the bracts of D.nobile for infestation,which can cause the bracts to be deformed and discolored,greatly reducing the ornamental value of D.nobile,and hence affecting its economic value.At the same time,the mature larvae of the pest can also drill into the soil to pupate,and can be spread for a long distance through trade of cut flowers and potted soil due to transportation.At present,this pest has caused serious economic losses to the Dendrobium industry in Okinawa,Fukuoka,Miyazaki,Hawaii and other places in Japan.The insects of family Cecidomyidae are traditionally identified mainly through morphology,which identification method is not accurate especially for the eggs and larvae of its similar species.An accurate and rapid identification of C.maculipennis Felt is hence needed to prevent its invasion into other Dendrobium nobile plantation areas in China.DNA barcoding technology was used to successfully identify the samples collected from a China(Sanya)International Orchid Show,Hainan Island as C.maculates Felt,and the morphological characteristics of C.maculates Felt were described in detail,which provided a scientific basis for accurate and rapid identification of C.maculates Felt by relevant quarantine departments.
NETL
NSTL
万方数据
